摘要
目的:研究PHEX抗体对小鼠牙胚发育的影响,探讨PHEX在牙齿发育过程的作用。方法:E17孕鼠尾静脉注射浓度分别为0.1mg/kg(A组)和0.5mg/kg(B组)的PHEX抗体。对照组N组注射0.1mol/LPBS。采用Mallory三色法染色观察钟状早期(E18)、钟状晚期(P1)、牙齿硬组织形成和矿化期(P3)牙胚的发育情况。免疫组织化学染色观察牙胚中PHEX蛋白的表达情况。结果:Mallory三色法染色结果显示:3组牙胚中釉质的发育无显著差异;成牙本质细胞的分化和牙本质的形成在A、N组中未见明显区别,而在B组中则明显延迟和抑制。E18、P1、P33组成釉细胞中的PHEX蛋白在牙胚中的表达均呈强阳性。P1、P3A组和N组中成牙本质细胞中的PHEX表达也呈强阳性,但B组在P1期靠近基底膜的牙乳头细胞中表达呈弱阳性,在P3期也仅有少量的弱阳性表达。结论:0.5mg/kg浓度的PHEX抗体可显著抑制成牙本质细胞的分化以及PHEX在其中的表达,进而抑制牙本质的生成;PHEX抗体对成釉细胞的发育和釉质的矿化无明显作用。
AIM : To investigate the effects of PHEX antibodies and possible roles of PHEX on the development of mouse tooth germ. METHODS: 0. 1mg/kg (group A) and 0.5mg/kg (group B) concentration of the PHEX antibody and 0.1M PBS (control group N) were injected into E17 pregnant rat through the tail vein. Samples of different stages, including early bell stage (E18), the late bell stage (P1) and dental hard tissue formation and mineralization stage (P3) were prepared and stained with Mallory's trichrome and immunohistochemical staining. RESULTS: Mallory trichrome staining results showed that there was no significant difference in the development of enamel in above three groups. No significant differences were also seen in the differentiation of odontoblasts and formation of dentin in group A and N. However, differentiation of odontoblasts and formation of dentin were significantly delayed and suppressed in group B. Expression of PHEX protein in ameloblasts of E18, P1, P3 were strongly positive in all three groups. Strong positive signals were also found in odontoblasts in P1, P3 of group A and N. But in the B group, weak positive PHEX signals were detected in dental papilla cells adjacent to the basement menbrane in P1 and in odontoblasts in P3. CONCLUSION: 0.5mg/kg concentration of PHEX antibody could significantly inhibited the differentiation and PHEX expression in odontoblasts, and thus inhibit the formation of dentin. PHEX antibodies could not effect the differentiation of ameloblasts and mineralization of enamel.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2009年第2期65-68,72,共5页
Chinese Journal of Conservative Dentistry
基金
教育部科学技术研究重点项目(JA06012)