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PHEX抗体对小鼠牙胚发育影响的实验研究

Effects of PHEX antibodies on the development of mouse tooth germ
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摘要 目的:研究PHEX抗体对小鼠牙胚发育的影响,探讨PHEX在牙齿发育过程的作用。方法:E17孕鼠尾静脉注射浓度分别为0.1mg/kg(A组)和0.5mg/kg(B组)的PHEX抗体。对照组N组注射0.1mol/LPBS。采用Mallory三色法染色观察钟状早期(E18)、钟状晚期(P1)、牙齿硬组织形成和矿化期(P3)牙胚的发育情况。免疫组织化学染色观察牙胚中PHEX蛋白的表达情况。结果:Mallory三色法染色结果显示:3组牙胚中釉质的发育无显著差异;成牙本质细胞的分化和牙本质的形成在A、N组中未见明显区别,而在B组中则明显延迟和抑制。E18、P1、P33组成釉细胞中的PHEX蛋白在牙胚中的表达均呈强阳性。P1、P3A组和N组中成牙本质细胞中的PHEX表达也呈强阳性,但B组在P1期靠近基底膜的牙乳头细胞中表达呈弱阳性,在P3期也仅有少量的弱阳性表达。结论:0.5mg/kg浓度的PHEX抗体可显著抑制成牙本质细胞的分化以及PHEX在其中的表达,进而抑制牙本质的生成;PHEX抗体对成釉细胞的发育和釉质的矿化无明显作用。 AIM : To investigate the effects of PHEX antibodies and possible roles of PHEX on the development of mouse tooth germ. METHODS: 0. 1mg/kg (group A) and 0.5mg/kg (group B) concentration of the PHEX antibody and 0.1M PBS (control group N) were injected into E17 pregnant rat through the tail vein. Samples of different stages, including early bell stage (E18), the late bell stage (P1) and dental hard tissue formation and mineralization stage (P3) were prepared and stained with Mallory's trichrome and immunohistochemical staining. RESULTS: Mallory trichrome staining results showed that there was no significant difference in the development of enamel in above three groups. No significant differences were also seen in the differentiation of odontoblasts and formation of dentin in group A and N. However, differentiation of odontoblasts and formation of dentin were significantly delayed and suppressed in group B. Expression of PHEX protein in ameloblasts of E18, P1, P3 were strongly positive in all three groups. Strong positive signals were also found in odontoblasts in P1, P3 of group A and N. But in the B group, weak positive PHEX signals were detected in dental papilla cells adjacent to the basement menbrane in P1 and in odontoblasts in P3. CONCLUSION: 0.5mg/kg concentration of PHEX antibody could significantly inhibited the differentiation and PHEX expression in odontoblasts, and thus inhibit the formation of dentin. PHEX antibodies could not effect the differentiation of ameloblasts and mineralization of enamel.
出处 《牙体牙髓牙周病学杂志》 CAS 北大核心 2009年第2期65-68,72,共5页 Chinese Journal of Conservative Dentistry
基金 教育部科学技术研究重点项目(JA06012)
关键词 PHEX 抗体 牙胚 Mallory三色法 免疫组织化学 PHEX antibodies tooth germ mallory trichrome staining immunohistochemistry
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  • 1吕红兵,吴甘茶,闫福华.小鼠牙胚发育过程中PHEX基因的时空表达模式研究[J].牙体牙髓牙周病学杂志,2008,18(2):65-68. 被引量:1
  • 2吴甘茶,闫福华,吕红兵.PHEX蛋白在小鼠成牙本质细胞分化过程中的定量表达[J].口腔医学研究,2008,24(2):145-147. 被引量:3
  • 3Fiona Franeis, Tim M Strom, Steffen Henning, et al. Genomic Organization of the Human Pex Gene Mutated in X - Linked Dominant Hypophosphatemie Rickets [ J]. Genome Res, 1997,7 (6) :573 -585.
  • 4Rowe PS. The PEX gene : its role in X - linked rickets, osteomalacia, and bone mineral metabolism [ J]. Exp Nephrol, 1997,5 (5) :355 - 363.
  • 5Boukpessi T, Septier D, Bagga S , et al. Dentin alteration of deciduous teeth in human hypophosphatemic rickets [ J ]. Calcif Tissue Int ,2006, 79 ( 5 ) :294 - 300.
  • 6秦满,石广香,葛立宏.低磷酸酯酶症乳牙的光镜和扫描电镜研究[J].中华口腔医学杂志,1999,34(4):220-222. 被引量:8
  • 7Boileau G, Tenenhouse HS, Desgroseillers L, et al. Characterization of PHEX endopeptidase catalytic activity : identification of parathyroid - houmone - related peptide 107 - 139 as a substrate and osteocalcin, PPi and phosphate as inhibitors [ J ]. Biochem J,2001, 355(3) :707 -713.
  • 8Camps M, Couture C, Hirata IY, et al. Human recombinant endopeptidase PHEX has a strict SI 'specificity for acidic residues and cleaves peptides derived from fibroblast growth factor- 23 and matrix extracellular phosphoglycoprotein [ J ]. Biochem J, 2003.373( 1 ) :271 - 179.
  • 9Row PS, Garrett IR, Schwarz PM, et al. Surface plasmom resonance (SPR) confirms that MEPE binds to PHEX via the MEPE ASARM motif: a model for inpaired mineralization in X - linked rickets (HYP) [J]. Bone,2005,36(1):33-46
  • 10Guo R, Liu S, Spuren.x RF, et al. Anah,sis of recombinant Phex: an endopeptidase in search of a substrate[ J ]. Am J Physiol Endocrinol Metab,2001,281 ( 4 ) :837 - 847

二级参考文献17

  • 1朱奇,于涌,樊明文,边专,陈智,彭彬.表皮生长因子受体在小鼠磨牙牙胚发育过程中的表达及其生物学意义[J].口腔医学研究,2006,22(4):360-363. 被引量:2
  • 2Hu C C,Pediatric Dentistry,1996年,18卷,17页
  • 3Fiona Francis, Tim M Strom, Steffen Henning, et al. Genomic Organization of the Human Pex Gene Mutated in X - Linked Dominant Hypophosphatemic Rickets [ J]. Genome Res, 1997, 7(6) :573 -585.
  • 4Qin C, Baba O, Butler WT. Post - translational modifications of sibling proteins and their roles in osteogenesis and dentinogenesis [J]. Crit Rev Oral Biol Med, 2004, 15(3) :126 - 136.
  • 5Turner AJ, Tanzawa K. Mammalian membrane metallopeptidases: NEP, ECE, KELL, and PEX [J]. FASEB J, 1997, 11 (5) :355 -364.
  • 6Boileau G, Tenenhouse HS, Desgroseillers L, et al. Characterization of PHEX endopeptidase catalytic activity: identification of parathyroid - hormone - related peptide107 - 139 as a substrate and osteocalcin, PPi and phosphate as inhibitors [ J ]. Biochem J, 2001,355(Pt3) :707 -713.
  • 7Row PS, Garrett IR, Schwarz PM, et al. Surface plasmon resonance (SPR) confirms that MEPE binds to PHEX via the MEPE -ASARM motif: a model for impaired mineralization in X - linked rickets (HYP) [J]. Bone, 2005, 36(1):33-46.
  • 8Matsumoto N, Jo OD, Shih RN, et al. Altered cathepsin D metabolism in PHEX antisense human osteoblast cells [ J ]. Biochem Biophys Res Commun, 2005, 332( 1 ) :248 -253.
  • 9Ruchon AF, Tenenhouse HS, Marcinkiewicz M, et al. Developmental expression and tissue distribution of Phex protein: effect of the Hyp mutation and relationship to bone markers [ J ]. J Bone Miner Res, 2000, 15(8) : 1440 - 1450
  • 10Thompson DL, Sabbagh Y, Tenenhouse HS, et al. Ontogeny of Phex/PHEXprotein expression in mouse embryo and subcellular localization in osteoblasts [ J ]. J Bone Miner Res, 2002, 17 (2) : 311 -320

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