大鼠相关脑区p-CREB和c-Fos在吗啡点燃条件性位置偏爱条件下的变化

Changes of p-CREB and c-Fos expression in brain regions of morphine-reactivated conditioned place preference in rats

目的:研究磷酸化cAMP反应元件结合蛋白(phospho-cAMP response element binding protein,p-CREB)和c-Fos在吗啡点燃条件性位置偏爱激活大鼠海马、杏仁核表达的变化。方法:以剂量递增连续皮下(s.c.)注射吗啡6 d建立吗啡诱导大鼠条件位置性偏爱(conditioned place preference,CPP)模型,第7天用生理盐水替代吗啡训练大鼠10 d,使CPP消退,单次s.c.吗啡(4 mg/kg)激发已消退的CPP。采用免疫组化技术测定吗啡激发CPP重现时大鼠海马、杏仁核p-CREB和c-Fos的变化。结果:吗啡可使大鼠产生CPP效应,吗啡4 mg/kg可使已消失的CPP效应激活;吗啡诱发CPP激活时大鼠海马、杏仁核p-CREB和c-Fos的表达增加。结论:海马、杏仁核p-CREB和c-Fos蛋白的表达参与了吗啡点燃CPP重现。

AIM： To investigate the changes of phospho-cAMP response element binding protein （p- CREB） and c-Fos in two brain regions of morphine-reactivated conditioned place preference （CPP） in rats. METHODS： Morphine was administered by subcutaneous injection at gradually increasing dose for 6 days to establish morphine CPP. From the 7th day, the rats were administered saline instead of morphine for 10 days to induce CPP extinction. The rats were given a single priming injection of morphine to reactivate the morphine CPP. The p-CREB and e-Fos were assayed with immunohistochemistry method in the phase of recurrence of CPP rekindled by morphine. RESULTS： Increasing dose of morphine conditioning for 6 days resuited in acquisition of CPP and morphine （4 mg/kg） reactivated CPP following 10 days drug free period. Compared with saline control, morphine -reactivated CPP elevated the expression of p-CREB and c-Fos in hippocampus and amygdala in rats （P 〈 0.05 ）. CONCLUSION： p-CREB and e-Fos expression in hippocampus and arnygdala may be involved in the mechanisms of recurrence of CPP.