用脉冲凝胶电泳和分子标记研究鲍曼不动杆菌菌株亲缘性

Molecular typing of Acinetobacter baumannii by genetic mark of drug-resistance gene and pulsed field gel electrophoresis

目的通过对宁波二院鲍曼不动杆菌菌株亲缘性的分析，对比耐药基因和脉冲场凝胶电泳（PFGE）分型方法。方法采用耐药基因扩增和PFGE技术研究我院临床分离的27株鲍曼不动杆菌基因型，对检测结果作菌株亲缘性聚合分析。结果27株鲍曼不动杆菌用PFGE分型分为4个亚型，用耐药基因分型分为2个亚型，聚合分析结论有区别。结论两种方法的适用范围不同，研究中选择合适的检测方法非常重要。

Objective To compare between genetic mark of drug-resistance gene and pulsed field gel electrophoresis（PFGE） by the analysis of the relations of Acinetobacter baumannii in Ningbo No.2 Hospital. Melhod The genetypes of 27 A. baumannii were analyzed by PFGE and drug-resistance gene by PCR. The relations were analyzed with cluster analysis methods. Results 27 strains of A. baumann/i were divided into two subgroups by drug-resistance gene PCR method and four subgroups by PFGE method. Cluster analysis showed the difference. Conclusions The two methods have different application range, so the correct mothod for detection is very much importance in the study.