摘要
目的:建立生物检材中氯胺酮及其代谢物去甲氯胺酮的检测方法,考察氯胺酮和去甲氯胺酮在大鼠体内的分布。方法:用高效液相色谱法(HPLC)检测生物检材中的氯胺酮及去甲氯胺酮;以10 mg·kg-1剂量给大鼠灌胃(ig),分别于给药后10 min,45 min,2 h和8 h处死,测定体液和组织中氯胺酮及去甲氯胺酮的含量。结果:建立起生物检材中氯胺酮及其代谢物去甲氯胺酮的HPLC法。所建方法在0.05-25.0μg.g-1浓度范围内线性关系良好,r2=0.999,萃取回收率大于75%;肝和肾中氯胺酮和去甲氯胺酮含量最高,其次为:脾、尿液、肺、脑、血液、心和肌肉。结论:所建方法准确、灵敏,适用于组织中氯胺酮及其代谢物去甲氯胺酮的分析鉴定;肝和肾可作为毒物分析的组织检材。
Objective：To establish the method for determination of ketamine and its metabolite norketamine in biological material and investigate the distribution of ketamine and norketamine in rats.Methods：The concentrations of ketamine and norketamine in biological samples were analyzed by high-performance liquid chromatography（HPLC）.Four groups of rats were fed with 10 mg·kg-1 of ketamine and killed at 10 min,45 min,2 h and 8 h post dose,respectively and determine the concentrations of ketamine and norketamine in biological fluids and tissues.Results：A HPLC method for the determination of ketamine and norketamine in biological samples were established.The method showed satisfactory linearity（r2=0.999） in a linear range from 0.05 to 25.0 μg·g-1.The extraction recovery was over 75%.Ketamine and norketamine concentrations of the administrated groups distributed in a degressive order of liver,kidney,spleen,urine,lung,brain,blood,heart,and muscle.Conclusion：The method is accurate,sensitive and suitable for the determination of ketamine and norketamine in tissues.Liver and kidney can be used as tissue samples for toxicological analysis of ketamine and norketamine.
出处
《中国药物依赖性杂志》
CAS
CSCD
2009年第1期28-33,共6页
Chinese Journal of Drug Dependence
基金
国家自然科学基金资助项目(30371577)
