不同消毒剂对毕氏海蓬子种子萌发和幼苗生长的影响

Effect of Different Disinfector on Seeds Germination and Seedlings Growth of Salicornia Bigelovii Torr.

研究不同消毒剂,84消毒液、升汞和双氧水对毕氏海蓬子种子和幼苗在不同激素种类及浓度配比的MS培养基上的长势、发芽率及抗污染率。结果表明,MS+BA1.0 mg/L+IAA 0.4 mg/L培养基最适宜海蓬子下胚轴及种子的生长。在此培养基上,体积分数为20%的84消毒液处理10 min对海蓬子下胚轴的消毒效果较好,其长势可达9 mm,抗污染率可达93%;质量分数为0.1%的升汞处理15 min对海蓬子种子的消毒效果较好,种子的发芽率最高可达90%,抗污染率可达97%。

Seeds or seedlings of salt-dilution halophyte Salieornia europaea were treated with different concentration of 84 disinfectors,mercuric chloride and hydrogen peroxide on MS medium with different concentration of hormone. The growth, germination rate and antipollution rate of S. bigelovii were studied in this paper. The results showed the best medium was MS with 1.0 mg/L BA ＋ 0.4 mg/L IAA. It is demonstrated that the disinfection effect of hypocotyl was best after treatment by the 84 disinfectors with the concentration of 20% for 10 minutes. The growth and the antipollution rate of S. bigelovii hypocotyl could achieve 9 millimeter and 93 percent respectively. And the disinfection effect of seeds was best after treatment by the mercuric chloride with the concentration of 0. 1% for 15 minutes. The germination rate and the antipollution rate of S. bigeiovii seeds could achieve 90 percent and 97 percent respectively.