原发性高血压患者sIL-2R及细胞免疫活性的变化

Number and the activity's change of sIL-2R and cell-mediated immunity in the essential hypertension

目的探讨ｓＩＬ－２Ｒ、Ｔ淋巴细胞亚群和ＮＫ细胞在原发性高血压中的变化与意义。方法分别用酶标双抗体夹心法、碱性磷酸酶抗碱性磷酸酶法和改良的微量细胞毒试验—乳酸脱氢酶释放法检测７６例原发性高血压患者的ｓＩＬ－２Ｒ、Ｔ淋巴细胞亚群、自然杀伤细胞（ＮＫ细胞）的数量与活性。结果原发性高血压患者ｓＩＬ－２Ｒ水平明显增高，ＣＤ３、ＣＤ４、ＣＤ４／ＣＤ８、ＮＫ细胞活性显著降低，且ｓＩＬ－２Ｒ和ＣＤ４／ＣＤ８的异常随病情的加重而加重。有家族史的患者较对照组ＮＫ细胞数量显著减少。结论原发性高血压患者存在ｓＩＬ－２Ｒ异常和细胞免疫的调节紊乱，终致ＩＬ－２的产生与功能障碍，此可能是引起本病的重要环节。ｓＩＬ－２Ｒ和ＣＤ４／ＣＤ８的异常变化可以反映疾病的严重程度，而ＮＫ细胞数量的异常似与本病的遗传因素有关。

Objective To research the changes and significancy of sIL-2R,T-lymphocyte and NK cells in patients with esstntial hypertension(EH).Method The alkaline phosphatase anti-aikaline phosphatase(APAAP),lactase dehydrogenase enzyme-release method,the sandwich enzyme-linked immunosorbent assay were used to study the numbers and activities of sIL-2R,T-lymphocyte and NK cells in the serum of 76 EH patients.Results The level of sIL-2R in EH patients' serum was significantly increased.The activities of CD 3,CD 4,CD 4/CD 8,NK cells in the EH patients' serum were lower than those in normal control group . The abnormality of sIL-2R and CD 4/CD 8 developed in patents with EH,the NK cells number was greatly decreased in patients with a family history.Conclusion Our study showed that abnormality of sIL-2R and cell laur immunity exhibited in EH patients.The reduction of production and activity of IL-2 are the important factors in EH immune damage.The increase of sIL-2R and decrease of CD 4/CD 8 can reflect the severity of the disease .The decrease of the NK cells number may relate with genetic factor of EH.