聚合链酶反应对海南疟区儿童带疟原虫的调查

CHILDREN INFECTED WITH MALARIA PARASITES DETECTED BY PRC METHOD IN THE EPIDEMIC AREA OF MALARIA IN HAINAN

为了解应用聚合酶链反应（PCR）检测疟区儿童带疟原虫的效果，参照文献合成恶性疟原虫（PF）与间日疟原虫（PV）引物各1对，并改进标本处理方法，按PCR检测操作常规，对采自乐东县的恶性疟与间日拒混合流行区的儿童血标本，分别进行PF与PV的PCR检测，同时以厚血膜镜检法对照比较。119例儿童血标本检测结果，PCR法与镜检法的带疟原虫率分别为6．72％与5．04％，镜检发现的PF与PV带虫者，PCR皆可拉出，PCR还检出3例镜检未发现的PF带虫者，显示PCR法比显微镜法的敏感性高，特异性强，适用于恶性疟与间日疟混合流行区人群的疟疾调查和监测。

In order to prove the effectiveness ofpolymerase chain reaction(PCR) method indetecting children infected with malaria par-asites in malaria epidemic areas, a pair ofprimers for both Plasmodium falciparum(PF ) and Plasmodium vivax (PV ) weresynthesized in reference to published litera-tures. With improved sample treatment,theblood samples from the children in theprevalence area of both pernicious malariaand malaria tertiana in Ledong County weretested by PCR routine procedures and com-pared with the results of the rouitine thickblood film examinations. The results of theblood examination of 119 children showedan infective rate of 6. 72% by PCR and itwas 5.04% by regular microscopy. Both PFand PV carriers detected by regular mi-croscopy were also detected by PCR testwhile 3 other PF carriers neglected by regu-lar microscopy were detected by PCR exam-ination It is suggested that PCR examina-tion is more sensitive,specific and applicablethan regular microscopy in the study andsurveillance of the people in prevalence ar-eas of both pernicious malaria and malariatertiana.