摘要
我们用鸟枪法，将普氏立克次体ＤＮＡ经酶切，连接，用ｐＵＣ１９为载体，大肠杆菌ＪＭ１０３为受体菌，将酶切后的连接产物转化到大肠杆菌ＪＭ１０３中去，用酶联法、改良补体结合法筛选克隆子。这样得到２株酶联效价为１∶１２８０的阳性克隆子，它们与普氏立克次体免疫血清呈阳性反应。
DNA obtained from the Breinl strain of Rickettsia Prowazedii and vector PUC19 DNA were digested with the restriction endonuclease Hind Ⅲ and linked with T4 DNA ligase.The linked mixture was used to transform E.Coli JM103 and plated on LB agar containing ampicillin.Bacterial clones were screened for antigen production with hyperimmune rabbit serum.Two of the clones revealed expression of antigen reactive with anti-R.Prowazekii serum.
出处
《疾病监测》
CAS
1998年第3期87-91,共5页
Disease Surveillance
