用驻极体技术改进脂质体包封血红蛋白制备的实验研究

Study on Improved Preparation of Liposomes Encapsulated Hemoglobin with Bioelectrets Technique

为改进脂质体包封血红蛋白（ＬＥＨ，即人工红细胞）制备技术，获得可输入实验动物体内的ＬＥＨ，以生物驻极体理论为指导，应用驻极体技术对ＬＥＨ进行极化处理，调整其驻极体特性，使其与生物体环境相适应。本研究制备四种经不同极化处理的ＬＥＨ。首先对四种ＬＥＨ应用热释电技术测试了它们的驻极体性能，表明将正极化后的Ｈｂ包封制成ＬＥＨ再行负极化得到的４号ＬＥＨ驻极体性能最好。其次对该种ＬＥＨ粘度及聚集性的观察，发现其粘度与人血相类似，而抗聚性要强于人红细胞。再次，观察了血液与４号ＬＥＨ不同配比对体外血栓形成的影响，结果表明血液中４号ＬＥＨ浓度为２０％，抑制血栓形成效果最优。最后依此浓度，观察了四种ＬＥＨ对体外血栓形成的影响，发现具有不同驻极体特性的ＬＥＨ对血栓形成影响各异。比天然人红细胞驻极体性能弱的１、２号ＬＥＨ，可促进体外血栓形成，表现出与人体血液驻极体性能的不匹配性。比天然人红细胞驻极体性能强的的ＬＥＨ可抑制体外血栓形成，表现出与人体血液驻极体性能良好的相容性，本项研究提示：（１）运用驻极体技术对ＬＥＨ进行调整，使其更适应生物体的生理环境，完善了ＬＥＨ制备技术，为进一步ＬＥＨ输注动物体内研究奠定了材料基础。（２）ＬＥＨ?

In order to improve the preparation technique of Liposome Encapsulated Hemoglobin(LEH), which cna be injected into animal, the bioelectrets technique was used for proceeded LEH with polarograph disposal, and adjusted the bioelectrets properties of LEH to adapt the biological surroundings. In this research, there were four types LEH to be prepared. First of all, the bioelectrects properties of four types LEH were studied with the Termally Stimulated Depolarization Current(TSDC). As a result, the experiment showed that the best bioelectrets properties which made of positive polarization inside and negative outside was No.4 LEH. Than, the further experiment about the viscosity and aggregation of NO.4 LEH had been done. The result showed that the NO.4 LEH had a similar viscosity with the human blood and was a higher aggregation than the human red blood cell. Also, the influence of the different density NO.4 LEH in human blood on thrombus formation was observed in vitro. he best inhibition effect can be observed when the concentration of NO.4 Leh in blood about 20%. According to this density, finally, the in fluence of four types of LEH on thrombus formation was observed in vitro. Theresults attended the different bioelectrets properties of LEH have different influence on thrombus formation in vitro. No.1 and No.2 Leh with weakness bioelectrets properties than naturally human red blood cell can promote the thrombus formation in vitro. In fact, it showed the matchless with bioelectrets properties of human red blood cell. No.3 and NO.4 LEH with strong bioelectrets properties than naturally human red blood cell, it can inhibited the thrombus formation in vitro. It showed the fine match with bioelectrets properties of human red blood cell. These study came to a conclusion: (1) make the technology of bioelectrets proceeded to LEH with polarograph disposal can improve the preparation technique of LEH. This method lay the groundwork to the study which LEH is injected to animal. (2) The study of LEH anti-thrombus was explored the possibility of the clinical application of bioelectrets. In one hand, it can explain the principle of physiology and pathology from the angle of bioelectrets. On the other hand, it also provided the clinical basis for intervened therapy of bioelectrets.