转移相关基因CD_(44V6)在喉癌中表达及意义

Expression of CD_(44V6) and its significance in laryngeal carcinoma

为探讨转移相关基因CD_(44V6)与喉鳞癌颈淋巴结转移和其他生物学行为的关系,采用免疫组化SP法对39例喉鳞状细胞癌进行标记分析,结果发现:CD_(44V6)阳性率,淋巴结转移组(88.24％)明显高于无淋巴结转移组(59.09％)(P<0.05),在喉鳞癌Ⅲ级(84％)高于鳞癌Ⅰ～Ⅱ级(50％)(P<0.05)。对CD_(44V6)阳性的组织切片研究发现,在某些切片中不是全部癌细胞均为CD_(44V6)阳性,有部分癌细胞为阴性反应。上述结果表明,在喉鳞癌组织内,存在不同潜能转移的癌细胞亚群,获得CD_(44V6)因子的癌细胞,具有转移的潜能,CD_(44V6)与喉鳞癌的低分化性,高浸润性及转移密切相关。

In order to evaluate the potential role of CD_(44) variant exon (CD_(44V6)) in laryngeal squamous cell carcinoma, CD_(44V6) expression in 39 paraffin-embeded laryngeal carcimoma tissues were studied by immunochemical SP method using antihuman CD_(44V6) monoclonal antibody. The results showed that positive immunoreaetivity of CD_(44V6) was detected in 16 of 17 cases (88.24%) with lymph node involvement and in 12 of 22 cases (59.09%) with no lymph node involvement, and it was also detected in 21 of 25 (84%) poorly differentiated carcinoma (Ⅲ) and in 7 of 14 (50%) well/moderately differentiated carcinoma (Ⅰ～Ⅱ). Additionally, in some positive staining slides of CD_(44V6), we found that some carcinoma cells were positive and some were negative. The results indicated that there were different potiential carcinoma cells in laryngeal squamous cell carcinoma, CD_(44V6) positive carcinoma cells had the metastatic potency, the expression of CD_(44v6) was closely correlated with lymph node metastasis and the differentiation of carcinoma cells.