耐甲氧西林金黄色葡萄球菌耐药性及erm基因的检测与分析

Drug-resistance and Gene erm Existing in Meticillin-resistant Staphylococcus aureus

目的了解耐甲氧西林金黄色葡萄球菌(MRSA)耐药性及erm基因存在状况。方法采用ATB Staph药敏试验板微量肉汤法对50株金黄色葡萄球菌(SAU)进行15种抗菌药物敏感性测定,采用聚合酶链反应(PCR)技术检测MRSA的erm基因。结果检出的42株MRSA对万古霉素、替考拉宁、夫西地酸和奎奴普汀/达福普汀均100.0%敏感;对青霉素、苯唑西林、庆大霉素、四环素、环丙沙星和左氧氟沙星100.0%耐药;42株MRSA中erm基因阳性的35株,阳性率为83.3%。结论临床分离的MRSA多药耐药性已十分严重;MRSA对红霉素的耐药机制主要是由erm基因编码甲基化酶,使药物作用靶位改变所致。

OBJECTIVE To investigate the resistance of meticillin-resistant Staphylococcus aureus （MRSA） and the occurrence of gene erm. METHODS ATB Staph and microdilute tests were performed to detect the susceptibility to 15 kinds of antibiotics in 50 strains of the S. aureus （SAU）. Gene erm of these strains was detected by polymerase chain reaction （PCR）. RESULTS There were no strains resistant to vancomycin, teicoplanin, fusidic acid and quinupristin-dalfopristin in 42 strains of MRSA detected. There were no strains sensitive to penicillin, oxacillin, gentamicin, tetracycline, ciprofloxacin and levofloxacin. Thirty-five strains habored gene erm in 42 strains of MRSA. The positive rate of gene erm was 83. 3%. CONCLUSIONS The multiple-resistance of the MRSA is a serious issue. The resistance to erythromycin in MRSA is mediated by gene erm which encodes the methylase and changes the target site of drug action.