阴沟肠杆菌中发现β-内酰胺酶基因新亚型blaLAP-2

bla_(LAP-2),A Novel Subtype of β-Lactamases Gene in Enterobacter cloacae in China

目的了解解放军第98医院临床分离的HZB9055号阴沟肠杆菌中常见耐药相关基因存在状况。方法于2004年5月从住院患者分离到HZB9055号菌株,采用聚合酶链反应及序列分析的方法分析41种(群)耐药基因。结果HZB9055号菌株共计检出3种基因,分别为blaLAP、blaMIR、qnrS,其余38种基因均阴性;blaLAP基因扩增序列全长含858个核苷酸,与窄谱β-内酰胺酶LAP-1(GenBank注册号:EF026092)相比,第193位氨基酸有不同,被命名为LAP-2(GenBank注册号:EU159120)。结论HZB9055号阴沟肠杆菌中至少存在3种耐药基因,其中blaLAP-2基因为一种新发现的β-内酰胺酶基因亚型。

OBJECTIVE To investigate the resistance genes in HZB9055 strain of Enterobacter cloacae isolated from the PLA 98th Hospital, Huzhou District,Zhejiang Province,China. METHODS HZB9055 Strain of E. cloacae was isolated from the inpatient in May, 2004,41 kinds （or groups） of genes were analyzed by PCR and verified by DNA sequencing. RESULTS In HZB9055 strain of E. cloacae, 3 kinds of genes were positive including blaLAP, blaMIR and qnrS. The 38 kinds of rest genes were all tested negative. The blaLAP gene sequence including 858 nucleotides, which had the amino acid mutation in position 193 compared with LAP-1 type narrow-spectrum β- lactamase （GenBank accession number： EF026092）, and was nominated LAP-2 （GenBank accession number： EU159120）. CONCLUSIONS At least 3 kinds of resistance genes exist in HZB9055 strain of E. cloacae, and blaLAP-2 is a novel subtype of β-1actamases gene.