产吲哚金黄杆菌和黏金黄杆菌产β-内酰酶的研究

Prevalence of Extended-spectrum Beta-lactamases and Metallo-beta-lactamses among Chryseobacterium indologenes and Ch.gleum

目的了解产吲哚金黄杆菌和黏金黄杆菌产β-内酰胺酶情况,特别是超广谱β-内酰胺酶和金属β-内酰胺酶。方法平板琼脂稀释法测定被试菌株对常用的12种抗菌药物的MIC;三维试验测定被试菌株的超广谱β-内酰胺酶和金属β-内酰胺酶;用3对引物扩增相应的基因。结果25株产吲哚金黄杆菌和10株黏金黄杆菌中,68.0%的产吲哚金黄杆菌和90.0%的黏金黄杆菌金属β-内酰胺酶阳性,但所有菌株中未发现超广谱β-内酰胺酶。结论金属β-内酰胺酶是产吲哚金黄杆菌和黏金黄杆菌多药耐药的主要原因。

OBJECTIVE To study the incidence of β-1actamases, mainly the extended-spectrum beta-lactamases （ESBLs） and metallo-beta-lactamases （MBLs） of Chryseobacterium indologenes and Ch. gleum. METHODS Agar dilution method was applied to detect minimal inhibitory concentrations （MIC） to 12 different antibiotics used frequently. Three-dimensional test was used to detect ESBLs and metallo-β-1actamases. The genes of β-lactamases were amplified with 3 pairs of primers special for Ch. indologenes and Ch. gleum. RESULTS Among the 25 strains of Ch. indologenes and 10 strains of Ch. gleurn, 68% （17/25） isolates of Ch. indologenes and 90% （9/10）isolates of Ch. gleum were considered as MBLs positive strains, hut no isolates were detected for the production of ESBLs. CONCLUSIONS MBLs are the important mechanism of multi-drug resistance for Ch. indologenes and Ch. gleum.