老年医院下呼吸道感染患者锌、铬与细胞免疫功能的变化

Changes in Trace Elements Zn and Cr and Cell Immunologic Function among Elderly Inpatients with Lower Respiratory Tract Infection

目的研究老年医院下呼吸道感染患者锌、铬与细胞免疫功能的变化。方法测定52例老年医院下呼吸道感染组患者血浆、红细胞内微量元素锌、铬与T细胞亚群,同时与53例健康老年组作对照;血锌测定用光焰原子吸收法,血铬测定用无光焰原子吸收法,T细胞亚群检测应用间接免疫荧光法。结果老年医院下呼吸道感染组血浆锌(12.68±3.26)μmol/L和铬(0.033±0.029)μmol/L、红细胞内锌(124.12±36.55)μmol/L和铬(0.412±0.025)μmol/L值较正常对照组(17.39±4.78)μmol/L和(0.068±0.036)μmol/L(、145.33±20.12)μmol/L和(0.666±0.038)μmol/L均下降,差异有统计学意义(均P<0.05);而且T细胞亚群显示功能减退及调节素乱的状况,即CD3(50.48±7.62)%、CD4(27.36±9.37)%及CD4/CD8(0.67±0.23)比值较正常对照组(64.33±4.82)%、(42.35±8.12)%及(1.57±0.22)%均有下降,而CD8(35.37±7.55)%较正常对照组(28.13±1.35)%增高,差异有统计学意义(均P<0.05)。结论某些老年医院下呼吸道感染患者存在着微量元素缺乏及细胞免疫功能障碍,有助于老年患者医院下呼吸道感染的防治。

OBJECTIVE To study the changes in trace elements Zn and Cr and cell immunologic function among elderly inpatients with lower respiratory tract infection. METHODS The changes in T-lymphocyte subpopulation and trace elements Zn and Cr in plasma and red blood cell of 52 inpatients with lower respiratory tract infection were compared with a control group of 53 healthy people of the same age group. The flame atom absorbing was for blood Zn testing,the non-flame atom absorbging for blood Cr testing the indirect immunofluorescent technique was for measurement of T cell subpopulation. RESULTS Among elderly inpatients with lower respiratory tract infection the ratio of Zn [（12.68±3.26）μmol/L] and Cr [（0. 033±0. 029）μmol/L] in plasma and Zn [（124. 12±36.55）μmol/L] and Cr [（0. 412±0. 025）μmol/L] in RBC was significantly lower than control group [（17.39± 4.78）μmol/L] and [（0. 068 ±0. 036）/μmol/L], [（145. 33 ± 20. 12）μmol/L] and [（0. 666 ± 0. 038）μmol/L], respectively, the function of T-lympholyte subpopulation was reduced and in disorder, CD3 [（50.48± 7.62）%], CD4[（27. 36±9. 37）% ]and CD4/CD8（0. 67 ± 0. 23） was significantly lower than control group [（64. 33 ± 4.82）%, （42.35±8.12）% and （1.57±0.22） %],respectively, while CD8 [（35.37±7.55） %] was significantly higher than control group [（28. 13 ± 1.35）%], the statistical differences were significant （P 〈 0. 05）. CONCLUSIONS Some elderly inpatients with lower respiratory trac infection lacking of trace elements and with cell immunity dysfunction, and the study result mentioned above will help to protect and treat the elderly inpatients with lower respiratory tract infection.