河北和山东鸭梨果实上链格孢菌鉴定

Identification of Alternaria isolates from Ya-pear fruits in Hebei and Shandong provinces

为了查清造成鸭梨果实储藏期黑斑病的链格孢Alternaria的种类,从其产孢表型、分生孢子特征和分子生物学等方面对分离的链格孢菌进行了研究。经对分离到的188支Alternaria菌株的形态学鉴定,共确定了3个种,即链格孢Alternaria alternata、细极链格孢A.tenuissima和侵染链格孢A. infectoria,比例分别为41.0%、54.8%和1.6%。分子生物学研究结果表明,Alternaria大孢子种彼此间及大孢子种与小孢子种之间可以根据ITS和gpd序列差异明确区分;而供试的多数小孢子种在ITS和gpd序列上差异很小,无法区分。

To identify the species of Alternaria, the pathogen that causes black spot on Ya-pears during storage, the isolated Alternaria from the sporulation pattern, conidia characters and molecular biology were examined. With morphological and molecular biological study on 188 isolated strains of Alternaria, three species are identified, namely Alternaria alternata, A. tenuissima, and A. infectoria, accounting for 41.0%, 54.8%, and 1.6% of the total strains respectively. Based on ITS and gpd data, it is evidently distinguished between the large-spored Alternaria spp. and between the large- and the small-spored Alter- naria spp. ; it is not distinguished among the samall-spored Alternaria spp.