重组人生长激素对烧伤大鼠肠源性脓毒症及其预后的影响

Influence of Recombinant Human Growth Hormone on Gut Derived Sepsis and Prognosis in Severely Scalded Rats

目的观察重组人生长激素(rhGH)早期应用对严重烧伤大鼠肠源性脓毒症、器官损害及其预后的影响。方法54只成年雄性Wistar大鼠随机分为对照组、烧伤组和GH组,后两组造成25%总体表面积Ⅲ度烫伤,立即腹腔注射地塞米松80 mg/kg,从伤后2 h开始连续3 d分别给予等渗盐水和rhGH[1.33 IU/(kg.d)];于伤后4、71、0 d,观察大鼠肝、脾、肾、肺、肠系膜淋巴结和门、腔静脉血液等组织脏器脓肿发生率、细菌检出率,肝、脾、肾、肺、肠等器官病理与功能改变,以及体重变化和10 d存活率等。结果GH组动物脏器脓肿发生率、细菌检出率和体重下降均明显低于烧伤组(P<0.05),器官结构与功能损害较轻,10 d存活率高于烧伤组。结论早期应用大剂量rhGH能有效降低严重烧伤后肠源性脓毒症发生率,减轻器官损害,提高存活率。

OBJECTIVE To investigate the influence of recombinant human growth hormone （rhGH） on gut derived sepsis, organ damage and prognosis in severely scalded rats. METHODS Fifty-four adult male Wistar rats were randomly divided into three groups, ie, control, burn and GH groups. The rats in burn and GH groups were inflicted with 25% total body surface area （TBSA） full-thickness skin scald on their backs and immediately followed by intraperitoneal injection of dexamethasone （80 mg/kg）. The scalded rats in the two groups were subcutaneously injected with normal saline and rhGH （1.33IU ·kg^-1·d^-1 ）, respectively, from 2 hours to 3 days after scalding. On dd 4, 7, 10 after scalding, the incidence of liver, spleen, kidneys, and lungs abscess, positive rate of bacteria culture of the organic tissues, mesenteric lymph nodes, and portal and cava venous blood, the pathological and functional change of the organs and intestine and the weight loss and 10 days survivor rate of scalded rats were observed. RESULTS The incidence of abscess, positive rate of bacteria culture, and weight loss in GH group were significantly lower than those in burn group （P〈0.05）. The morphology and function of the organs were obviously better than that in burn group. The 10 days survivor rate in GH group was higher than that in burn group. CONCLUSIONS rhGH administered in early stage after severely burning could effectively decrease the incidence of bum-induced gut-derived sepsis and alleviate the organ damage, which could contribute to the higher survivor rate.