大、小肝癌DNA含量测定及多项生物学指标的对比分析研究

A Study on DNA Content and Tumor Biological Characteristics of Large and Small Hepatocellular Carcinoma

应用图像分析技术，对18例小肝癌和38例大肝癌行DNA含量测定，并分析其肿瘤生物学特性，结果显示小肝癌DNA含量以低倍异倍体为主（66．7％），大肝癌以高倍异倍体为主（94．7％），P<0．01，在肿瘤包膜突破和癌栓形成率上小肝癌（11．1％和22．2％）明显低于大肝癌（P<0．01），小肝癌存在明显的不典型增生，预后优于大肝癌，而病理分级，肝硬变程度两组无显著差异。提示＜3cm的小肝癌基本反映了早期肝癌的肿瘤生物学特征，是获得根治性切除的良好时机。DNA含量测定是估计肝癌恶性程度和临床预后的准确和客观的指标。

DNA content was quantitatively determined in 18 small hepatocellular carcinoma(small HCC) and 38 large HCC by image analysis technique. The result showed that low aneuploidDNA content were found in 66.7% of small HCC and high aneuploid were found in 94.7% of largeHCC (p<0.01). The incidences of tumor capsule invasion and cancerous thrombus formation were11. 1 % and 22. 2 % in small HCC which were significantly lower than in large HCC (p<0.01 ). Hepatocellular dysplasia was more evident in small HCC. The prognosis was better in small HCC. It issuggested that <3cm small HCC basically reflects the tumor biological characteristic of early HCCand is an important opportunity for achieving radical resection. The quantitatively analysis of DNAcontent is a precise and objective reference for evaluating tumor biologic feature and prognosis ofHCC.