以恶臭假单胞菌发酵制备D-氨基葡萄糖酸

Production of D-Glucosaminic Acid by Fermentation with Pseudomonas putida

从土样中分离到D-氨基葡萄糖酸高产菌株GNA5,综合Biolog细菌自动鉴定系统与16SrDNA序列分析结果,鉴定为恶臭假单胞菌(Pseudomonas putida).P.putidaGNA5能耐受较高浓度底物D-氨基葡萄糖,可发酵积累D-氨基葡萄糖酸.初步优化培养基为(g/L):D-氨基葡萄糖盐酸盐30,葡萄糖5,尿素5,KH2PO42,MgSO4-7H2O0.5,CaCO310,pH7.0.菌株GNA5在该培养基中发酵48h可制备25.5g/L的D-氨基葡萄糖酸,摩尔转化率达93.91%.自行筛选的P.putidaGNA5显示了底物转化能力强及产物分解能力弱的特点,具有进一步产业化开发的潜力.

Strain GNA5 producing D-glucosaminic acid from D-glucosamine was isolated from soil samples. It was identified as Pseudomonas putida based on the assay of Biology Automated Microbiology Identification System and analysis of 16S ribosomal DNA sequence. P. putida GNA5 could grow in the medium containing high concentration of D-glucosamine, and D-glucosaminic acid was stably accumulated in the oxidative fermentation process. The preliminary optimized culture medium composition （g/L） for the production of D-glucosaminic acid with P. putida GNA5 was obtained as follows： D-glucosamine-HCl 30, glucose 5, urea 5, KH2PO4 2, MgSOa.7H2O0.5, CaCO3 10, pH 7.0. After 48 h fermentation in this medium, the concentration of D-glucosaminic acid reached 25.5 g/L, with molar yield of 93.9%. P. putida GNA5 exhibited efficient conversion ability of D-glucosamine to D-glucosaminic acid and low degradation ability of D-glucosaminic acid. It shows considerable economic significance in development of D-glucosaminic acid producing process.