全反式维甲酸对涎腺腺样囊性癌细胞株Acc-M体外抗增殖作用的研究

EFFICACY OF ALL TRANS RETINOIC ACID IN INHIBITION OF HUMAN SALIVARY ADENOID CYSTIC CARCINOMA CELL PROLIFERATION IN VITRO

目的维甲酸类化合物能预防头颈部癌前病变转变为癌，并能有效地降低头颈部第二原发癌的发生率。近来研究证实，维甲酸对头颈部鳞癌有诱导分化治疗作用，但维甲酸对头颈部腺癌诱导分化作用研究少见报道。方法本研究采用克隆形成法、ＭＴＴ检测法以及细胞周期测定技术，把全反式维甲酸（ａｌｔｒａｎｓｒｅｔｉｎｏｉｃａｃｉｄＡ－ＴＲＡ）对人涎腺腺样囊性癌细胞株（ＡｃｃＭ）的体外抗增殖作用和细胞增殖周期进行了观察。结果显示，ＡＴＲＡ对ＡｃｃＭ细胞有一定抗增殖作用，且该作用与浓度、时间呈依赖关系；ＡｃｃＭ细胞经ＡＴＲＡ作用７２小时后其Ｓ期比例明显下降，同时Ｇ０＋Ｇ１期比例上升。结论研究表明，５～２０μｍｏｌ／Ｌ浓度的ＡＴＲＡ对ＡｃｃＭ细胞产生抗增殖作用。

Retinoids are proved to prevent carcinogenesis in head and neck premalignant lesions and to prevent second primary carcinoma in patients with head and neck cancer. Recent studies have demonstrated the efficacy of retinoic acid in inducing differentiation therapy of carcinoma. In the present study, all trans retinoic acid was tested for its antiproliferative activity aganist human salivary adenoid cystic carcinoma cell(AccM) in vitro. Clonogenic assay and MTT assay as well as cell cycle analysis were used in this experiment. These observations showed that ATRA can inhibit the growth of AccM cell with does and time response manner. The decrease in proportions of cells in Sphase was detected following 72 h of treatment, this was accompanied by an increase of cell in G0 and G1. These results indicated that proliferation of AccM can be inhibited by higher concentrations of ATRE.