阿托伐他汀对血管紧张素Ⅱ诱导的人血管内皮细胞炎性因子的影响

Effect of atorvastatin on expression of inflammatory factor induced by Ang Ⅱ in vascular endothelial cells

目的观察阿托伐他汀对血管紧张素Ⅱ(Ang Ⅱ)诱导的人脐静脉内皮细胞(HUVECs)单核细胞趋化因子(MCP-1)和NF-κB抑制因子IκB-α蛋白表达的影响。方法无菌取新生儿脐带,体外培养HUVECs,取3～5代细胞加入AngⅡ10^(-6)mol/L为实验组,不加培养液为对照组,分别培养2、4、6、10、12 h。另取HUVECs,分为培养组(仅加培养液)、AngⅡ组(加AngⅡ)、阻断剂组(加AngⅡ和NF-κB抑制剂)、药物组(加AngⅡ和阿托伐他汀),每组6例,培养12 h,采用硝酸还原酶法测定NO含量,蛋白印迹法检测MCP-1和IκB-α蛋白表达。结果实验组各时间点NO含量均低于对照组(P<0.01).而MCP-1蛋白表达则显著高于对照组(P<0.01),且呈时间依赖性。阻断剂组和药物组NO含量较AngⅡ组均明显升高(P<0.01),但仍低于培养组(P<0.01);MCP-1蛋白表达较AngⅡ组均减少,但高于培养组(P<0.01)。与AngⅡ组比较,阻断剂组和药物组IκB-α蛋白表达显著升高(P<0.01),但仍低于培养组。结论阿托伐他汀对AngⅡ诱导的内皮细胞损伤具有保护作用。

Objective To observe the effect of atorvastatin on Ang Ⅱ-induced expression of monocyte chemoattractant protein-1 （MCP-1） and IκB-α in human umbilical vein endothelial cells （HUVECs）. Methods Umbilical cord of newborn infant was taken aseptically. After cultivation, the 3rd-Sth generations of the cells were taken,and Ang Ⅱ was added to their nutritive medium for cultivating for 2,4,6,10 and 12 h. There was control at every time point. After that, other HUVECs were taken and divided into four groups：control, Ang Ⅱ , blocker （Ang Ⅱ and NF-κB inhibitors were added）and drug（Ang Ⅱ and atorvastatin were added）. After 12 h, content of NO, protein expression of IκB-α and MCP-1 were observed. Results Compared with control,content of NO was lower and protein expression of MCP-1 was higher at every time point in experiment groups,which showed time dependence. After 12 h,content of NO in blocker and drug groups was higher than that in Ang Ⅱ group, but lower than control （P 〈 0.01） ;protein expression of MCP-1 in these two groups was lower than Ang Ⅱ group （P 〈0. 01） and higher than control （P 〈 0.01）. Comparing the two groups,there was no significant difference. After 12 h, protein expression of IκB-α in blocker and drug groups was higher than that in Ang Ⅱ group （P 〈 0.01）,but lower than that in control （P 〈0.01）. Conclusion Atorvastatin can protect the damaged endothelium caused by Ang Ⅱ.