3种药物对鼠黑素瘤细胞系B16F10黑素合成和细胞增殖的影响

Effects of three drugs on proliferation and melanogenesis of B16F10 melanoma cell

目的研究氨茶碱、前列腺素E1和雌激素是否能够影响B16F10鼠黑素瘤细胞的生物活性并探讨其可能的机制。方法将上述3种药物各配制成4种不同的浓度加入到RPMI-1640培养液中与B16F10黑素瘤细胞共同培养。结果氨茶碱在40 mg/L、160 mg/L的较高浓度时、前列腺素E1在0.01～0.64 mg/L、雌二醇在0.025～1.6 mg/L范围内对细胞增殖均有刺激作用(P<0.05或<0.01);氨茶碱于10～160 mg/L、前列腺素E1在0.01～0.64 mg/L、雌二醇在0.025～1.6 mg/L范围内对酪氨酸酶均有激活作用(P<0.05或<0.01);氨茶碱在40 mg/L、160 mg/L的较高浓度时、前列腺素E1在0.04～0.64 mg/L、雌二醇在0.025～1.6 mg/L范围内对黑素合成均有显著的促进作用(P<0.05或<0.01)。结论雌激素、前列腺素E1及较高浓度的氨茶碱均能对B16F10鼠黑素瘤细胞的细胞增殖、酪氨酸酶活性和黑素合成产生刺激作用。

Objective To investigate whether aminophylline, prostaglandinE1 （PGE1） and estrogen could affect biological activity of melanocyte and the possible mechanism. Methods Aminophylline, PGE1 and estradiol were diluted into four different concentrations respectively. B16F10 melanoma cells were cultured in RPMI-1640 medium with these drugs. Results The cell proliferation rate was increased by aminophylline with the concentrations of 40 μg/ml, 160 μg/ml, PGE1 0.01 - 0.64 μg/ml, and estradiol 0. 025 - 1.6 μg/ml. The activity of tyrosinase was improved by aminophylline with the concentrations ranging 10 - 160 μg/ml, PGE10. 01 - 0.64 μg/ml, and estradiol 0. 025 - 1.6 μg/ml. Mdanogenesis was promoted by aminophylline with the concentrations of 40 μg/ml, 160 μg/ml, PGE1 0.04 μg/ml- 0.64 μg/ml, and estradiol 0.025 - 1.6 μg/ml. Conclusion PGE1 and estradiol as well as aminophylline with high concentrations can stimulate B16F10 melanoma cell proliferation, improve tyrosinase activityand promote melanogenesis.