益气化瘀胶囊调控冠状动脉粥样硬化斑块形成的实验研究

Investigation of Yiqihuayu capsule regulation the formation of atherosclerotic plaque

目的探讨益气化瘀胶囊调节血脂,特别是降低低密度脂蛋白胆固醇(LDL-C)以及调控冠状动脉粥样硬化斑块形成的效果及机理。方法将80只大鼠分为4组(模型组、实验组、对照组以及正常组);模型组、实验组、对照组喂饲高脂饲料造模,实验组同时予益气化瘀胶囊喂饲,对照组同时予辛伐他汀胶囊喂饲。采用酶法测定总胆固醇(TC)、LDL-C、高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG);采用组织病理学常规染色技术(苏木精-伊红法)和特殊染色技术(苏丹Ⅲ法)检测冠状动脉以及肝、肾、脑组织的病理改变。结果实验组、对照组、正常组调节血脂和调控冠状动脉粥样硬化斑块形成以及对脂质在肝脏代谢的影响结果与模型组比较差异均有统计学意义(P<0.01)。结论益气化瘀胶囊通过益气活血化瘀通脉的作用,可有效调节血脂代谢,特别是降低血清LDL-C的含量,控制延缓或逆转冠状动脉粥样硬化的进程。

Objective To investigate the mechanism of the Yiqihuayu Capsule＇s regulating blood lipid, especially its decreasing LDL- C and its regulating the formation of coronary atherosclerosis. Methods （1） To make animal models with 80 male Wistar rats by feeding feed high - fat food and divided into 4 gwoups （AS model control group, Yiqihuayu Capsule test group, Simvastatin tablets control group, normal control group）. （2） To investigate the mechanism of the Yiqihuayu Capsule＇s regulating blood lipid and regulating the formation of Coronary Atherosclerotic by biochemical detection and histopathological observation. （3）To determinate TC, LDL- C, TG with enzyme method. To detect the pathological change of coronary artery, liver, kidney and brain by histopathological routine staining technique （Methods Hematoxylin Eosin （HE）） and special staining technique （Sudan Ⅲ ）. Results There were significant difference between Yiqihuayu Capsule test group, Simvastatin tablets control group, normal control group and As model control group on regulating effects to the formation of coronary atheroosclerotic and liver lipid metabolism （ P 〈 0.01 ）. Conclusion Yiqihuayu capsule can effectively regulated blood lipid metabolism and decrease the content of serum LDL - C, and effectively delay or reverse the course of coronary atherosclerotic, so prevent the occurrence of coronary heart disease.