纤维素酶高产担子菌株的筛选鉴定及酶学性质初探

Screening and identification of basidiomycete from cellulase and preliminary study on its enzymatic properties

从河北省棉田土壤样中分离出一株纤维素酶高产菌株，经中科院微生物所鉴定，初步确定为一株担子菌（a basidiomycetous specie），将其命名为担子菌LKY01。对该菌株酶学性质进行了初步探索。研究表明，本酶反应最适pH值为5．0左右；最适反应温度约60℃；Co^2＋、Mn^2＋对本酶酶活有明显的抑制作用，Cu^2＋、Fe^2＋、Ca^2＋、Na^＋、K^＋、Sr^2＋、Mg^2＋对本酶有一定的促进作用；在50℃下本酶有较好的稳定性，其酸碱稳定范围在pH4．0～8．0。

A strain of high - producing cellulase was screened from the cotton field soil sample in Hebei province, and it was identified as a basidiomycetous specie denominated LKY01 based on Institute of Microbiology, CAS. The enzymatic properties of the strain was researched. The optimum reaction temperature and pH of enzyme were 60℃ and 5.0, respectively. The enzymatic activity was restrained by Co^2＋ ,Mn^2＋, but enhanced by Cu^2＋,Fe^2＋ , Ca^2＋ , Na ^＋ ,K ^＋ ,Sr^2＋ , Mg^2＋. The enzymatic activity was stable below 50℃, between pH 4.0 and pH 8.0.