内皮素和血小板活化因子在急性胰腺炎发病机制中的意义

The Significance of Endothlin and Platelet-Activating Factor in Mechanism of Acute Pancreatitis

目的探讨血浆内皮素(Endothelin,ET)血清血小板活化因子(Platelet-activating-Factor,PAF)在急性胰腺炎(Acute Pancreatis,AP)发病中的作用及意义。方法将60只大鼠分假手术组(F组)、AP组,采用放射免疫分析法及ELISA法监测各组中不同时段(6、12、24h)血ET、PAF水平,并观察胰腺组织病理学改变。结果与对照组相比,AP组随时间、病变进展血ET、PAF水平逐渐升高,胰腺病理评分明显增高。结论随着急性胰腺炎病情的加重血ET水平逐渐升高,PAF水平早期明显增高,晚期下降。

Objective To explore the effects of endothelin （ET） and platelet-activating factor （PAF） in the pathogenesis and the clinical significance in acute pancreatitis （AP）. Methods Sixty rats were divided into the fake operated group （Normal Control Groups） and AP group, the levels of ET and PAF were tested by the analysis methods of radioimmunoassay and ELISA at different time - points （the 6th, 12th , 24th hour treatment）, and the pathologic change of the pancreas was observed by light microscope. Results Compared with the fame operated groups, the levels of AP group increased according as exacerbation of AP. The levels of ET and PAF increased, the histopathologic score of panereatitis was increased significantly. Conclusion The levels of AP group increased according as exacerbation of AP. The levels of ET increased, the levels of PAF significantly increased in early period and declined in late period.