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氟苯尼考人工抗原的合成与鉴定 被引量：1

Synthesis and identification of florfenicol antigen
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摘要 采用混合酸酐(MA)法,将氟苯尼考(FFC)分别与牛血清白蛋白(BSA)和卵清蛋白(OVA)连接,制备人工免疫抗原FFC-HS-BSA和包被原FFC-HS-OVA,经紫外扫描(UV)、凝胶电泳(SDS-PAGE)、红外扫描光谱(IR)和动物免疫试验证实人工抗原合成成功。 The immunogen, FFC-HS-BSA （florfenicol-bovine serum albumin）, and the coating antigen FFC-HS- OVA （florfenicol-ovalbumin） were synthesized by using the mixed anhydride method. The man-made antigens were identified and certified by UV absorption, SDS-PAGE electrophoresis and animal immunization test. The results showed the process was successful. All antigens made had the correct haptens and carrier proteins.
作者 孙法良 刁有祥 刘悦竹 孙宁 马艳芳
机构地区 山东农业大学动物科技学院 山东立鼎生物技术研究所
出处 《福建农业学报》 CAS 2009年第1期24-28,共5页 Fujian Journal of Agricultural Sciences
基金 山东省自然科学基金项目(Y2007D32)
关键词 氟苯尼考 人工抗原 混合酸酐法 凝胶电泳 Florfenicol synthesized antigen mixed anhydride method gel electrophoresis
分类号 S851 [农业科学—预防兽医学]
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参考文献11

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二级参考文献66

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福建农业学报
2009年 第1期

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