农药厂不同生境中甲胺磷降解细菌的分离、筛选与鉴定

Isolation,screening and identification of methamidoghos-degrading bacteria from habitats at a pesticide factory

对福建省福农生化有限公司不同生境中的甲胺磷降解细菌进行分离,用磷脂脂肪酸分析方法对菌进行鉴定,同时对降解细菌的生境特征进行分析。结果表明:对甲胺磷有降解作用的细菌共有22个属、28个种、67株菌株;在沟中污泥中分离到的降解细菌有8个属、12个种、35株菌株,其种类和数量多于其他的生境;而在各类降解细菌中,芽孢杆菌属和假单胞菌属分别有21株和19株,占分离到细菌总量的59.7%,其中的蜡状芽孢杆菌(Bacillus cereus)和恶臭假单胞菌(Pseudomonas putida)在以上3种生镜中大量存在。

To study methamidoghos-degrading bacteria in different habitats at a pesticide factory, soil samples from sewage, drainage ducts and surroundings at Fu-Nong Biochemistry Co. , Ltd in Fujian were collected for the isolation. Sixty-seven bacterial stains were isolated employing media containing high concentration of methamidoghos followed by identification using the fatty-acid analysis. They were determined to belong to 22 genera. In the 3 different types of habitats, 35 microcial stains belonging to 8 genera were found. The numbers of Bacillus＇and Pseudomonas strains were 21 and 19, respectively. In combination, they amounted to 59.7% of the total. Among them, Bacillus cereus and Pseudomonas putida could grow in soils of all the sampling sites.