肠激安方对腹泻型肠易激综合征模型大鼠T淋巴细胞和5-羟色胺的影响

Effect of Changji'an Prescription on T Lymphocytes and 5-Hydroxytryptamine in Rats with Diarrhea Predominant Irritable Bowel Syndrome

【目的】观察肠激安方对腹泻型肠易激综合征(IBS)模型大鼠T淋巴细胞水平和5-羟色胺(5-HT)分泌的影响。【方法】选用新生SD大鼠46只,随机分成正常组,模型组,肠激安方低、中、高剂量组(中药低、中、高剂量组,剂量分别为0.94、1.88、3.76 g.kg-1.d-1),补脾益肠丸组(剂量为6.76 g.kg-1.d-1);采用直肠内醋酸刺激法复制肠道高敏感大鼠模型,经大鼠排便统计及肠道敏感性评估确认造模成功后,采用流式细胞术检测各组外周血CD3+、CD4+、CD8+T淋巴细胞亚群水平,采用免疫组化法观察各组大鼠肠组织5-HT阳性细胞数和免疫染色强度。【结果】模型组大鼠排便量显著增加,肠道敏感性显著升高,CD4+水平及CD4+/CD8+比值显著降低,CD8+水平显著升高,5-HT阳性细胞数及染色强度显著增加(均P<0.01);中药低、中、高剂量组可减少大鼠排便量(治疗第14天),降低肠道敏感性,升高CD4+水平及CD4+/CDS+比值,降低CD8+水平,减少5-HT阳性细胞数及染色强度(均P<0.01)。【结论】肠激安方治疗IBS的作用可能与改善细胞免疫功能和肠道敏感性,减少5-HT分泌有关。

Objective To observe the effect of Changji＇an Prescription （CP） on T lymphocytes count and 5- hydroxytryptamine （5-HT） secretion in rats with diarrhea predominant irritable bowel syndrome （IBS）. Methods Forty-six neonatal SD rats were randomized into normal group, model group, low-, middle- and high-dose CP groups （0. 94, 1.88 and 3.76 g·kg^-1·d^-1 respectively）, and Bupi Yifei Pills group （6.76 g·kg^-1·d^-1 ）. Continuous internal rectal stimulation with acetate was used to induce the rat model of intestinal hypersensitive IBS. Flow eytometer was used for the quantitative analysis of peripheral levels of T lymphocytes subsets CD3^＋ , CD4^＋ and CD8^＋ , and immunohistochemieal method was used to observe 5-HT-positive cells number and the immunostaining intensity in the bowels of rats . Results In the model group, the defecation volume and the intestinal sensitivity were increased, CD4^＋ level and CD4^＋/CD8^＋ decreased, CDs level increased, 5-HT-positive cells number and the immunostaining intensity elevated （P 〈 0.01 compared with the normal group）. The defecation volume 14 days after treatment and the intestinal sensitivity were decreased, CDf level and CD4^＋/CD8^＋ increased, CDs level decreased, 5-HT-positive cells number and the immunostaining intensity lowered in CP groups （P 〈 0. 01 compared with the model group）. Conclusion The therapeutic mechanism of CP for IBS is probably related with the improvement of cellular immunity and intestinal sensitivity, and with the reduction of 5-HT secretion.