不同海拔COPD血浆中抗碳酸酐酶抗体水平

The level of plasma anti-CA Ⅱ antibody in the patients of Chronic Obstructive Pulmonary Disease at different altitude area

目的通过检测不同海拔地区正常组和慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)组人血浆中抗碳酸酐酶(carbonic anhydrase,CA)Ⅱ抗体滴度,分析它与红细胞数、动脉血二氧化碳分压(PaCO2)和肺功能的关系,探讨抗CAⅡ抗体在高原COPD发生、发展中的作用。方法选择高海拔、中度海拔与低海拔地区COPD患者和相应海拔正常对照组共6组168例,每组均用酶联免疫吸附法(ELISA)测定抗CAⅡ抗体滴度,并行血气分析和肺功能等检查。结果高原COPD组和高原对照组抗CA抗体滴度低于中度海拔COPD组和中度海拔对照组,后者低于平原COPD组和平原对照组,均有显著意义(P<0.01);但高原COPD组与高原正常对照组比较,无显著意义(P>0.05);抗CA抗体与红细胞数呈负相关(P<0.01),与PaCO2呈正相关(P<0.01),与肺功能呈负相关(P<0.01)。结论高原地区COPD患者和正常人抗CAⅡ抗体表达降低。

Objective To study the role of anti -carbonic anhydrase （CA） Ⅱ antibody in the occurrence and progression chronic obstructive pulmonary disease （COPD） by observing human plasma anti-CA Ⅱ antibody and analyzing the relationship among it and red blood cell and arterial partial pressure of carbon dioxide （ PaCO2 ） and lung function in control and COPD groups at different altitude area. Methods There are 6 group including 89 COPD patients and 79 normal control at high and medium and low altitude area, per group was tested the anti-CA Ⅱ antibody by Elisa technique and blood air anlysis and lung function etc examination. Results The anti-CA Ⅱ antibody of COPD groups and control in high altitude area were lower than that in medium altitude area,the latter were lower than that in champaign altitude area, which were significant（ P 〈 0. 01 ） ;but the anti-CA Ⅱ antibody for COPD and the controls in high altitude area werecompared, which was unsignificant（ P 〉 0. 05 ）. The relationship between anti-CA Ⅱ antibody and red blood cell and was negatively correlated （ P 〈 0. 01 ） ,the relationship between the antibody and PaCO2 was positively correlated （P 〈0. 01 ）, The relationship between the antibody and lung function was negatively correlated（P 〈 0. 01 ）. Conclusion It is low for the expression of the anti-CA Ⅱ antibody of COPD patients and control in high altitude area.