组织激肽释放酶基因与2型糖尿病关系的研究

Relationship between the polymorphism of the regulatory region of human tissue kallikrein gene and type 2 diabetes mellitus

目的初步探讨组织激肽释放酶基因调控序列启动子多态性与中国人2型糖尿病的相关性。方法应用PCR技术结合等位基因特异寡核苷酸片段分析(ASO)方法,对30例2型糖尿病患者和40例正常人的组织激肽释放酶基因用A、B、C、D、E、F、H、I、K、P 10种探针检测,比较组间的等位基因频率差异,初步分析该SNP位点与2型糖尿病的关系。结果组织激肽释放酶基因启动子A、B、H、K型在2型糖尿病组中的分布频率分别为46.67%、35.0%、15.0%、3.33%,在对照组中分别为45.0%、37.5%、17.5%、0%,两组比较有差异,但无统计学意义。结论在中国汉族人群中,该位点确实有多态性,A、B、H、K型均有分布,以A型最广。

Objective To study the association of the single nucleotide polymorphism （SNP） in the promoter region of tissue kallikrein gene with type 2 diabetes mellitus （DM）. Methods Ten alleles were detected with polymerase chain reaction and allele specific oligonucleotide analysis （ASO） in 30 patients with DM and 40 healthy subjects. Results The frequencies of A, B, H and K alleles in DM （46.67%, 35%, 15% and 3.33%, respectively） was no statistical difference from that of subjects （45%, 37.5%, 17.5% and 0%, respectively） （P〉0. 05）. Conclusion A, B, H and K alleles of tissue kallikrein gene are four types of polymorphism in Han nationality.