摘要
应用生物信息学的方法对GenBank中拟南芥、火炬松、胡麻、紫花罗兰等植物香豆酸-3-羟基化酶(c3h)基因以及本实验室克隆的欧美杨107的c3h基因(AM920690)核苷酸序列以及翻译的氨基酸序列的组成成分、导肽、跨膜结构域、疏水性/亲水性、蛋白质二级结构及功能域等进行了初步分析预测。分析表明欧美杨107的C3H氨基酸序列不存在导肽,无跨膜结构域,肽链表现为亲水性;二级结构域与P450高度匹配,其高级结构中含有由含铁血红素和半胱氨酸组成的活性中心,并构建了c3h进化树,对其分子进化进行了探讨。
The nucleic acid sequences and amino acid sequences of coumarate 3- hydroxylase from Pirats taeda, Arabidopsis thaliana, Sesamum indicure and Ocimum basilicurn which registered in GenBank and the c3h gene amplified by our laboratory from poplar（ Populus x euramericana ov.＂74/ 76＂）developing second xylem mRNA（accession numbers： AM920690） were analyzed and predicted by the tools of bioinformatics. The results showed that the amino acid sequences of c3h gene we amplified has no leader peptides and trails- membrane topological structure,it has the same secondary structure and functional domains of protein with cytochrome P450, and also has a active center with ferrous haemachrome and cysteine, Phylogenetic tree was constructed based on c3h genes from different plants, and its molecular evolution was also analyzed.
出处
《生物信息学》
2009年第1期13-17,共5页
Chinese Journal of Bioinformatics
基金
辽宁省自然科学基金(20062146)
辽宁省教育厅科学研究项目(20040091)
