摘要
研究了在乙酸-乙酸钠缓冲液中用复合酶(淀粉酶/糖化酶)水解玉米淀粉的工艺条件,对影响水解液DE值的主要因素进行了探讨。结果表明,影响DE值的主要因素依次为温度、pH值、酶比例(α-淀粉酶:糖化酶)、时间。通过正交实验得到优化工艺条件为:酶比例为1:4,温度为60oC,pH值为4.0,时间4h。在优化条件下,水解液DE值为64.21。
Enzyme complex （α-amylase/glucoamylase） was used for catalysis of native corn starch hydrolysis in acetic acid-sodium acetate buffer. Results showed that the degressive order of the influential factors affecting the DE value was temperature, pH, ratio of complex enzyme and the reaction time. The optimum ct-amylase-glucoamylase ratio, temperature, pH, and reaction time were l：4, 60 ℃, 4.0, and 2h, respectively, under which the DE value was up to 64.21.
出处
《现代食品科技》
EI
CAS
2009年第3期302-304,共3页
Modern Food Science and Technology
关键词
复合酶
DE值
玉米淀粉
水解
complex amylolytic enzyme
DE value
com starch
hydrolysis
