两套鉴别品种对云南稻瘟病菌株鉴别能力的比较

Comparison of Differentiating Capacity of Two Sets of Differential Lines Using Isolates of Magnaporthe grisea Collected from China’s Yunnan Province

【目的】比较日本鉴别品种和国际水稻研究所(IRRI)以中国普感品种丽江新团黑谷为回交亲本选育的水稻近等基因品系的鉴别能力。【方法】应用云南省3个稻作区27个县(市)采集、分离的418个稻瘟病菌分生孢子单孢菌株,分析两套水稻鉴别品种的鉴别能力,两套材料各由9个携带不同主效抗稻瘟病基因的品种组成,一套为日本鉴别品种,另一套为2个日本鉴别品种和7个以普感品种丽江新团黑谷为背景的近等基因系(IRBL鉴别品系)组成。【结果】以普感品种丽江新团黑谷为回交亲本选育的IRBL鉴别品系有较高的鉴别能力。IRBL鉴别品系可鉴定出126个小种,而日本鉴别品种鉴定出的小种数仅为95个。【结论】使用遗传背景一致,主效抗病基因不同的鉴别品种是较为理想的,有较高的鉴别能力。

[Objective] The objective was designed to compare the differentiating capacity of two sets of differential lines. [ Method ] A total of 418 blast monoconidial isolates collected from 27 counties of Yunnan Province were tested using two sets of differential varieties which hold nine different resistance genes. One set is Japanese differential varieties, another set contained two Japanese differential varieties and seven monogenic lines which developed using susceptible variety Lijiangxintuanheigu （LTH） as recurrent parent （IRBL lines）. Both of them hold the same set of resistance genes. The differentiating capacity of IRBL lines to Yunnan isolates was compared with that of Japanese differential varieties. [Result] The result showed that the differentiating capacity of IRBL lines was better than Japanese differential varieties to rice blast fungus colleted from Yunnan. These monoconidial isolates were divided into 126 races based on the reactions to IRBL lines, while they were divided into 95 races using Japanese differential varieties. [Conclusion] The result indicated that the monogenic differential varieties with same genetic background are better than that of complex background.