期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

应用三维建模方法定量分析C57BL/6J小鼠耳蜗内毛细胞带状突触的数量 被引量：6

原文传递
导出
摘要 哺乳动物中耳蜗内毛细胞带状突触(ribbon synapse)是外界声音信号向大脑听中枢传递路径上的第一个感觉神经突触结构,它在声音的编码和神经递质的释放过程中发挥着重要作用.然而由于带状突触的数量相对比较少,而且空间位置深在,所以仅应用电子显微镜观察的方法定量分析其数量始终在技术上存在一定的困难.本研究通过对C57BL/6J小鼠耳蜗基底膜标本带状突触中的特异突触前结构RIBEYE和非特异突触后结构GluR 2&3同时进行免疫荧光标记,采用激光共聚焦显微镜对标本进行光学连续切片,利用3DS MAX软件对连续切片标本进行三维建模,结果中每个荧光色对代表一个突触的存在.在此基础上对内毛细胞带状突触的数量进行计数,结果显示,耳蜗内毛细胞带状突触的空间分布及数量均可以清晰完整的重现出来.在本实验中,每个内毛细胞带状突触数量平均为(16.10±1.03)个.本实验结果表明,利用免疫荧光对小鼠耳蜗带状突触的前后膜结构进行双重标记,采用激光共聚焦显微镜对标本进行光学连续切片,应用3DS MAX软件对连续切片进行三维建模后可以准确计数带状突触的数量.实验表明,本方法准确可行,对今后深入探讨感觉神经性聋的机制具有重要意义。
作者 柳柯 李姝娜 姜学钧
机构地区 中国医科大学附属第一医院耳鼻咽喉科
出处 《中国科学（C辑）》 CSCD 北大核心 2009年第2期187-192,共6页 Science in China(Series C)
关键词 内毛细胞 带状突触 激光共聚焦显微镜 三维建模
分类号 R764 [医药卫生—耳鼻咽喉科]
  • 相关文献

参考文献23

  • 1李姝娜,姜学钧,柳柯,任重.内毛细胞带状突触的形态及功能研究进展[J].中华耳鼻咽喉头颈外科杂志,2008,43(5):391-394. 被引量：7
  • 2Safieddine S, Wenthold R J. SNARE complex at the ribbon synapses of cochlear hair cells: analysis of synaptic vesicle-and synaptic membrane-associated proteins. Eur J Neurosci, 1999, 11:803-812.
  • 3Francis H W, Rivas A, Lehar M, et al. Two types of afferent erminals innervate cochlear inner hair cells in C57BL/6J mice. Brain Res, 2004, 1016:182-194.
  • 4Fuchs P A. Time and intensity coding at the hair cell's ribbon synapse. J Physiol, 2005, 566:7-12.
  • 5Merchan-perez A, Liberman M C. Ultrastructural differences among afferent synapses on cochlear hair cells: correlations with spon-taneous discharge rate. J Comp Neurol, 1996, 371:208-221.
  • 6Schnee M E, Lawton D M, Furness D N, et al. Auditory hair cell-afferent fiber synapses are specialized to operate at their best frequencies. Neuron, 2005, 47:243-254.
  • 7Khimich D, Nouvian R, Pujol R, et al. Hair cell synaptic ribbons are essential for synchronous auditory signalling. Nature, 2005, 434: 889-894.
  • 8Liberman M C. Morphological differences among radial afferent fibers in the cat cochlea: an electron-microscopic study of serial sections. Hear Res, 1980, 3:45-63.
  • 9Kiang N Y, Rho J M, Northrop C C, et al. Hair-cell innervation by spiral ganglion cells in adult cats. Science, 1982, 217:175-177.
  • 10Spoendlin H. Anatomy of cochlear innervation. Am J Otolaryngol, 1985, 6:453-467.

二级参考文献28

  • 1Schnee ME, Lawton DM, Furness DN, et al. Auditory hair cell afferent fiber synapses are specialized to operate at their best frequencies. Neuron, 2005, 47 : 243-254.
  • 2Stamataki S, Francis HW, Lehar M, et al. Synaptic alterations at inner hair cells precede spiral ganglion cell loss in aging C57BL/6J mice. Hear Res, 2006, 221: 104-118.
  • 3Fuchs PA. Time and intensity coding at the hair cell's ribbon synapse. J Physiol, 2005, 566: 7-12.
  • 4Francis HW, Rivas A, Lehar M, et al. Two types of afferent terminals innervate cochlear inner hair cells in C57BL/6J mice. Brain Res, 2004, 1016: 182-194.
  • 5Spassova MA, Avissar M, Furman AC, et al. Evidence that rapid vesicle replenishment of the synaptic ribbon mediates recovery from short-term adaptation at the hair cell afferent synapse. J Assoc Res Otolaryngol, 2004, 5: 376-390.
  • 6tom Dieck S, Altrock WD, Kessels MM, et al. Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex. J Cell Biol, 2005, 168: 825-836.
  • 7Vahnte C, Spano S, Luini A, et al. Purification and functional properties of the membrane fissioning protein CtBP3/BARS. Methods Enzymol, 2005,404: 296-316.
  • 8Sakaba T, Stein A, Jahn R, et al. Distinct kinetic changes in neurotransmitter release after SNARE protein cleavage. Science, 2005, 309 : 491-494.
  • 9Sudhof TC. The synaptlc vesicle cycle. Annu Rev Neurosci, 2004, 27 : 509-547.
  • 10Yasunaga S, Graft M, Cohen-Salmon M, et al. A mutation in OTOF, encoding otoferlin, a FER-like protein, causes DFNB9, a nonsyndromic form of deafness. Nat Genet, 1999, 21 : 363-369.

共引文献6

同被引文献77

引证文献6

二级引证文献20

中国科学（C辑）
2009年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部