中国HIV-1 B／C重组毒株慢性期感染者Pol特异性T淋巴细胞反应的研究

Pol-specific T lymphocyte responses in Chinese HIV-1 recombinant subtype B/C chronically infected persons

目的:研究中国主要流行的HIV-1B/C重组毒株慢性期感染者Pol特异性T淋巴细胞反应特征并确定主要识别的免疫优势区域。方法:本研究以25名HIV-1B/C重组毒株慢性期感染者为研究对象,应用酶联免疫斑点试验(Elispot)测定技术综合测定了针对覆盖HIV-1Pol基因的249条重叠多肽产生γ干扰素的特异性T淋巴细胞免疫反应。结果:25名HIV-1B/C重组毒株慢性期感染者中有15(60%)名检测到反应,主要识别位于逆转录酶区、氨基酸位置为Pol241至295内的Pol5521、Pol5525、Pol5526、Pol5531四条多肽和Pol708至722内的Pol5638多肽。慢性期感染者分泌γ干扰素的特异性T淋巴细胞反应强度与病毒载量呈现明显正相关(P=0.00695,r=0.660)。结论:中国HIV-1B/C重组毒株慢性期感染者主要识别逆转录酶区氨基酸位置为Pol241至295和Pol708至722的区域。

Objective：To analyze the characteristics of Pol-specific T lymphocyte responses and identify immunodominant regions recognized in Chinese HIV-1 recombinant subtype B/C chronically infected persons. Methods： Twenty-five Chinese HIV-1 recombinant subtype B/C chronically infected persons were enrolled. HIV-l-specific T lymphocyte responses directed against 249 overlapping peptides spanning HIV-1 Pol gene were analyzed by an IFN-γ Elispot assay. Results： Pol-specific T lymphocyte responses of IFN-γ secretion were identified in 15 of 25 （60%） chronically infected persons. The specific T lymphecytes were mainly targeted at four peptides whose amino acid positions were from Pol 241 to 295 ： Pol5521, Pol5525, Pol5526, Pol5531 ,and another peptide,i, e. Pol5638 whose amino acid positions were from Pol 708to 722 in the reverse-transcriptase region. There was a positive correlation between the magnitude of Pol-specific IFN-γ secretion T lymphocyte responses and plasma viral load（P = 0. 00695, r = 0.660）. Conclusion： Chinese HIV-1 recombinant subtype B/C chronically infected persons mainly recognized regions whose amino acid positions were from Pol 241 to 295 and Pol 708 to 722 of reverse-transcriptase.