丁酸钠对结肠癌细胞SW480凋亡及Survivin基因和VEGF表达水平的影响

Effects of Sodium Butyrate on the Expression of Survivin and VEGF,and on the Apoptosis of SW480 Colon Carcinoma Cells

目的:观察丁酸钠对结肠癌细胞株SW480的生长抑制、凋亡情况以及对生成素(Survivin)表达水平的影响,探讨其预防结肠癌的作用机制。方法:人传代结肠癌细胞株SW480经不同浓度丁酸钠处理后,在不同时段收集细胞,分别用四唑蓝法检测肿瘤细胞生长增殖率,流式细胞仪检测细胞周期和凋亡变化,免疫细胞化学技术观察对Survivin基因和血管内皮生长因子(VEGF)表达水平。结果:随着培养液中丁酸浓度的不断升高和培养时间的延长,SW480细胞的生长逐渐受到抑制。G1期细胞明显增多,S期细胞明显减少,细胞凋亡率逐渐增高。Sur-vivin基因和VEGF随着丁酸浓度的不断升高而表达下降。结论:丁酸钠能抑制SW480细胞株增生,诱导凋亡,并抑制Survivin基因和VEGF表达。

Objective： To study the effect of sodium butyrate on the growth and apoptosis of SW480 colon carcinoma cells and the expression of survivin gene, and to explore their mechanisms in colon cancer prevention. Methods： The subculture of human colon cancer cell line SW480 was treated with different concentration of butyrate. The cells were collected after different time of cultivation. The cell proliferation rate and apoptosis were measured by MTT and flow cytometer, meanwhile, immunochemistry were used to observe the expression of survivin gene and VEGF （vascular endothelial growth factor） in SW480 cell line after treated by sodium butyrate. Results： By increasing the butyrate concentration and culturing time, the proliferation of SW480 cell was inhibited gradually. The percent of G1 stage cell was increased gradually whereas the percent of S stage cell was decreased. The apoptosis rate of SW480 was increased gradually at the same time. By increasing the butyrate concentration, the expression of survivin and VEGF decreased gradually. Conclusion： Sodium hutyrate can inhibit the expression of survivin and VEGF, and induce the apoptosis of SW480 cell line.