摘要
目的:探讨核因子κB(NF-κB)的亚单位RelA反义寡核甘酸对人细胞滋养细胞基质金属蛋白酶(MMP)-2,-9表达的影响。方法:离体培养人早孕期细胞滋养细胞(CTB),将反义和正义RelA寡核酸片断分别瞬时转染CTB,采用逆转录-聚合酶链反应(RT-PCR)和蛋白印迹(Westernblot)法检测基质金属蛋白酶-2,-9的mR-NA和蛋白表达。结果:CTB转染RelA反义寡核甘酸后,MMP-9的mRNA和蛋白的水平均下降(P<0.05),而MMP-2mRNA和蛋白水平均无显著变化(P>0.05)。结论:RelA反义寡核甘酸可以调节细胞滋养细胞MMP-9的表达,提示RelA可能通过调节MMP-9的表达参与胚泡着床过程中滋养细胞对子宫内膜的侵袭。
Objective: To explore whether RelA subunit of nuclear factor-κB (NF-κB) regulates the expression of MMP-2,-9 in human cytotrophoblastic cells (CTB) from the first trimester placenta in vitro. Methods: Human CTB from the first trimester placenta were cultured in serum free. Antisense and sense RelA oligonucleotide was transiently transfected into CTB respectively. The expression of MMP-2 and MMP-9 was detected by RT-PCR and Western blot. Results: Transient transfection of antisense ReIA oligonucleotide into CTB inhibits the expression of MMP-9 both in transcriptional and translating levels (P〈0. 05). While expression of MMP-2, unlike MMP-9, remained unchangable when transfected with antisense ReIA oligonucleotide (P〉0.05). Conclusion: The results suggest that RelA can regulate the expression of MMP-9 in human CTB from the first trimester placenta in vitro, and may consequently regulate the invasion process dur ing embryo implantation.
出处
《武汉大学学报(医学版)》
CAS
北大核心
2009年第2期177-179,183,共4页
Medical Journal of Wuhan University