RelA反义寡核甘酸对人细胞滋养细胞基质金属蛋白酶-2,-9表达的影响

Effects of Antisense RelA Oligonucleotide on the Expression of Matrix Metalloproteinase-2 and-9 in Human Cytotrophoblastic Cells in Vitro

目的:探讨核因子κB(NF-κB)的亚单位RelA反义寡核甘酸对人细胞滋养细胞基质金属蛋白酶(MMP)-2,-9表达的影响。方法:离体培养人早孕期细胞滋养细胞(CTB),将反义和正义RelA寡核酸片断分别瞬时转染CTB,采用逆转录-聚合酶链反应(RT-PCR)和蛋白印迹(Westernblot)法检测基质金属蛋白酶-2,-9的mR-NA和蛋白表达。结果:CTB转染RelA反义寡核甘酸后,MMP-9的mRNA和蛋白的水平均下降(P<0.05),而MMP-2mRNA和蛋白水平均无显著变化(P>0.05)。结论:RelA反义寡核甘酸可以调节细胞滋养细胞MMP-9的表达,提示RelA可能通过调节MMP-9的表达参与胚泡着床过程中滋养细胞对子宫内膜的侵袭。

Objective： To explore whether RelA subunit of nuclear factor-κB （NF-κB） regulates the expression of MMP-2,-9 in human cytotrophoblastic cells （CTB） from the first trimester placenta in vitro. Methods： Human CTB from the first trimester placenta were cultured in serum free. Antisense and sense RelA oligonucleotide was transiently transfected into CTB respectively. The expression of MMP-2 and MMP-9 was detected by RT-PCR and Western blot. Results： Transient transfection of antisense ReIA oligonucleotide into CTB inhibits the expression of MMP-9 both in transcriptional and translating levels （P〈0. 05）. While expression of MMP-2, unlike MMP-9, remained unchangable when transfected with antisense ReIA oligonucleotide （P〉0.05）. Conclusion： The results suggest that RelA can regulate the expression of MMP-9 in human CTB from the first trimester placenta in vitro, and may consequently regulate the invasion process dur ing embryo implantation.