建立人喉鳞癌裸鼠移植模型两种方法的比较

Comparison of Two Methods of Establishing Transplantation Tumor Model of Nude Mice with Human Larynx Squamous Carcinoma

目的:建立人喉鳞癌(Hep-2)裸鼠移植模型,比较两种建模方法成瘤效果。方法:将裸鼠随机分成两组,每组8只,两组分别于皮下接种人喉鳞癌细胞悬液和改良法皮下接种瘤块建立动物模型,观察接种成瘤率、肿块大小、生长速度;免疫组化检测人端粒酶逆转录酶(hTERT)蛋白表达。结果:接种3d后,改良瘤块接种法组裸鼠可见皮下隆起瘤体,接种1周后,皮下接种瘤细胞法和改良瘤块接种法成瘤率分别为75%、87.5%,比较第1周瘤体体积,P<0.05,但成瘤后肿瘤生长方面无明显的差别(比较第3周瘤体体积,P>0.05),两种方法成瘤后的瘤块hTERT蛋白表达阳性率相同。结论:采用改良瘤块接种法成瘤率高,生长速度快,且裸鼠皮下肿瘤较好的继承了人喉鳞癌细胞的重要生物学特点,该法能较好建立了人喉鳞癌裸鼠移植模型,为后续以端粒酶为靶点的体内抗肿瘤研究奠定良好基础。

Objective： Objective： To compare the two methods of establishing a nude mice transplantation tumor model with human larynx squamous carcinoma. Methods： The nude mice were randomly assigned into two groups to receive injection of Hep-2 carcinoma cell suspension and modified injection of tumor masses subcutaneously, to establish the nude mice model with human larynx squamous carcinoma. The successful rate of the tumor transplantation, the size of tumors, and growth speed were observed; the hTERT expression level in transplantation tumor tissue was measured by immunohistochemical method. Results： The models of modified injection of tumor masses had emerged smaller tumor three days later. After one week, the successful rate of the tumor transplantation by injection carcinoma cell suspension was 75%, while the rate by modified injection of tumor masses was 85%, meanwhile, the size of transplantation tumors was different between the two groups too （both P〈0. 05）. Three weeks later, no differences were found in the size of transplantation tumors and in the rate of hTERT expression between the two groups. Conclusion： Construction of larynx squamous careioma tumor model in nude mice by modified injection of tumor masses is better than the way of injecting cell suspension, and the subcutaneously tumor could inherit some significance biology features of larynx squamous carcio ma, thus paves a way for further investigation on telomerase, an antitumor target, in vivo.