骨桥蛋白在硬皮病患者皮损中的表达和意义

Expression of Osteopontin in Skin Lesions of Patients with Scleroderma and Its Significance

目的:探讨骨桥蛋白(OPN)在硬皮病患者局部皮损表达和分布情况。方法:采用免疫组化SABC法分别检测硬皮病皮损20例(系统性硬皮病12例,局限性硬皮病8例)和正常皮肤10例中OPN蛋白的表达情况。结果:OPN蛋白在硬皮病患者皮损真皮层的成纤维细胞中表达明显增高,而正常对照组仅少数呈微弱表达(P<0.01),其在系统性硬皮病患者和局限性硬皮病患者之间表达无明显差异(P>0.05)。结论:OPN蛋白在硬皮病患者皮损成纤维细胞内明显高表达,提示OPN可能参与了硬皮病的发病机制,对纤维化的形成发挥一定的作用,并对局限性和系统性硬皮病有相似的作用机制。

Objective： To explore the expression and distribution of osteopontin （OPN） in local lesions of scleroderma patients. Methods： The expression of OPN was examined by immunohistochemical analysis in serial sections of scleroderma biopsies obtained from 20 patients （12 systemic scleroderma, 8 local scleroderma） and 10 normal controls. Results： Significant increase of OPN expression in fibroblasts was found in skin lesions of patients with scleroderma compared to that in normal controls （P〈0. 01）. However, OPN expression in systemic scleroderma showed no significance difference compared to that in local scleroderma （P〉0. 05）. Conclusion： High expression of OPN in skin lesions of patients with scleroderma suggests that OPN might take part in the pathogenesis and have an important role in the fibrosis of scleroderma.