HIF-1α、VEGF在家族性息肉病中的表达及相关性研究

Expression of HIF-1 Alpha and VEGF in Familial Adenomatous Polyposis and Their Correlation

目的:探讨缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)在家族性腺瘤性息肉病(FAP)患者结肠腺瘤、癌变腺瘤中的表达变化及相关性,分析其对家族性腺瘤性息肉病"腺瘤-腺癌"发生发展的作用。方法:应用免疫组化SP法分别检测10例家族性腺瘤性息肉病患者术后切除标本中正常上皮、≤0.5cm腺瘤、≥1.0cm腺瘤以及癌变腺瘤中HIF-1α和VEGF表达情况,并分析二者与临床病理特征之间关系。结果:在正常上皮黏膜、≤0.5cm腺瘤、≥1.0cm腺瘤以及癌变腺瘤变化过程中两者表达逐渐增强(P均大于0.05),其表达与腺瘤体积有关,癌变腺瘤与正常黏膜之间表达有显著差异(P<0.05)。≤0.5cm腺瘤与正常黏膜之间无显著差异,但癌变腺瘤与两者之和有显著差异(P<0.05)。两者表达水平呈现极显著正相关(P<0.01)。结论:HIF-1α、VEGF在家族性腺瘤性息肉病"腺瘤-腺癌"发展中起重要作用,HIF-1α、VEGF表达存在显著的正相关,表明在家族性腺瘤性息肉病"腺瘤-腺癌"进程中,VEGF的表达主要由HIF-1α水平调控。

Objective： To analyze the expression and relationship of hypoxia inducible factor-1 alpha （HIF-1α） and vascular endothelial growth factor （VEGF） in patients with familial adenomatous polyposis （FAP） and to discuss their possible role in adenoma formation and cancerization. Methods. The adenoma specimens were obtained from 10 FAP patients （year 2005-2007）. Normal tissues,adenomas at different sizes （40.5 cm or ≥1 cm） and cancerous adenomas samples were subjected to immunohistochemical SP technique for analysis of HIF-1α and VEGF expression. Results.. When adenomas was ≥1.0 cm in size,the HIF-1α and VEGF expression in adenomatous epithelial cells was stronger than in normal epithelial cells （P〈0.05）. The expression of HIF-1α and VEGF in cancerous adenomas was much higher than that in normal tissues and in 40.5 cmadenomas tissues （P〈0.05）. There is a significant positive correlation between the expression of HIF-1α and VEGF （P〈0. 01）. Conclusion： HIF-1α may play a role in adenoma-carcinoma sequence via regulation of VEGF.