E-cadherin表达缺失在白血病细胞行为中的作用

Influence of absent expression of E-cadherin on leukemic cell behaviors

目的:确定上皮-钙黏蛋白(E-cadherin)表达缺失在白血病细胞行为中的作用,最终阐明E-cadherin介导的骨髓微环境与造血细胞相互作用改变在白血病发生中的作用。方法:细胞黏附实验测定HL-60细胞经5-氮杂脱氧胞苷(5-Aza-CdR)诱导E-cadherin表达后细胞黏附能力的改变,MTT法测定细胞增殖能力,细胞迁移实验确定细胞的迁移能力。结果:HL-60细胞经5-Aza-CdR诱导E-cadherin表达后,其对E-cadherin-Fc的黏附能力增加,细胞在E-cadherin-Fc上的增殖能力下降,细胞黏附导致细胞迁移能力下降。结论:白血病细胞中由于E-cadherin表达丧失,引起细胞黏附降低,增殖能力增强,细胞迁移活跃,表明E-cadherin表达缺失与白血病细胞行为的获得有关。

AIM： To investigate the influence of absent expression of E - cadherin on leukemic cell behavior and further determine the role of abnormal interactions between hematopoietic progenitor and bone marrow on leukogenesis. METHODS ： After E - cadherin was induced with 5 - Aza - CdR in HL - 60 leukemic cells, cell adhesion ability was determined by cell adhesion assay. E - cadherin mediated adhesion associated proliferation was examined by MTF assay, cell mobility was determined by a transwell migration assay. RESULTS ： After E - cadherin was induced by 5 -Aza- CdR in HL- 60 cells, the adhesion ability of cells with E -cadherin -Fc was enhanced. E -cadherin mediated adhesion then inhibited HL -60 cells proliferation and suppressed cell migration ability. CONCLUSION： Absent expression of E - cadherin on leukemic cells plays a role in the decreased adhesion, enhanced proliferation and increased migration ability of leukemic cells. Loss of E - cadherin expression is responsible for the behaviors of leukemia cells.