摘要
目的:研究大肠肿瘤组织中Bmi-1蛋白表达情况及其与大肠癌临床病理特征及预后的关系,并探讨Bmi-1蛋白在大肠癌中的表达与Ki67蛋白表达的关系。方法:采用免疫组织化学方法分别检测Bmi-1蛋白在60例大肠癌、30例大肠腺瘤及20例正常大肠黏膜组织3组中的表达情况及其与大肠癌临床病理特征及患者生存率的关系,并探讨大肠癌中Bmi-1蛋白表达与Ki67蛋白的相关性。应用SPSS13.0软件包对结果进行统计学分析。结果:Bmi-1蛋白在大肠癌、大肠腺瘤及正常大肠黏膜组织中的表达率分别为25.0%、6.7%、0%,Bmi-1蛋白在大肠癌中表达明显高于腺瘤组及正常组(P<0.05),而在腺瘤组及正常组中的表达差异无显著(P>0.05);Bmi-1蛋白高表达与有无远处转移及TNM分期密切相关(P<0.05),而与患者性别、年龄、肿瘤大小、分布部位、分化程度、组织类型及淋巴结转移等临床病理特征无关(P>0.05);Kaplan-Meier生存分析显示Bmi-1蛋白高表达患者生存率明显低于低表达患者(P<0.05);大肠癌中Bmi-1蛋白高表达与Ki67蛋白表达无相关关系(P>0.05)。结论:Bmi-1蛋白表达与大肠癌的发生、转移及预后关系密切,可作为评估患者侵润转移及预后的参考指标。
AIM： This study was to investigate the expression and significance of Bmi - 1 in colorectal carcinoma （ CRC）, and to explore the effect of Bmi - 1 on Ki67 expression in human CRC. METHODS ： The samples from sixty CRC, thirty adenomas and twenty normal colorectal mucosal tissues were used in this study. The expression of Bmi - 1 protein was detected by immunohistochemistry. The clinicopathological features and survival rate of patients were also analyzed. RESULTS ： The overexpression of Bmi - 1 was respectively 25.0%, 6. 7% and 0% in CRC and adenomas as well as normal colorectal mucosal tissues. The results showed that the expression of Bim - 1 was significantly higher in CRC, compared with that in adenomas and normal colorectal mucosal tissues （ P 〈 0.05 ）. The overexpression of Bmi - 1 protein in CRC was obviously associated with distant metastasis and TNM stage （P 〈0. 05）, but not with gender, age, tumor size, tumor site, histological type, differentiation degree and lymph node metastasis （P 〉 0.05）. Kaplan - Meier survival analysis showed that the overexpression of Bmi - 1 reduced significantly survival of CRC patients （ P 〈 0.05 ）. No statistical rela- tion between expression of Bmi - 1 and Ki67 in CRC was observed. CONCLUSION ： The overexpression of Bmi - 1 protein is significantly correlated with tumorigenesis, metastasis and prognosis of CRC. Bmi - 1 might be regarded as a parameter in evaluating prognosis of CRC.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2009年第3期497-501,共5页
Chinese Journal of Pathophysiology
基金
广东省科技攻关资助项目(No.2006B35502009)
