NF-кB在Graves病免疫病理机制中的意义

Significance of NF-кB in immunopathogenesis of Graves disease

目的:探讨Graves病(GD)患者外周血单个核细胞(PBMCs)NF-кB活性变化及其在GD免疫病理机制中的意义。方法:分别采集22例未经治疗的GD患者(未经治疗组)、20例经他巴唑治疗>1年的GD患者(治疗组)及25例健康志愿者(对照组)静脉血,采用凝胶电泳可动性移位分析(EMSA)检测PBMCs中NF-кB活性;采用放免法检测血清IL-1β、IL-6及TNF-α含量。结果:未经治疗GD组患者PBMCs NF-кB活性显著高于治疗组及正常对照组(P<0.05);未经治疗GD组患者血清IL-1β、IL-6、TNF-α水平明显高于治疗组和正常对照组(P<0.05);未经治疗GD组和治疗组NF-кB活性与IL-6水平均呈正相关。结论:GD患者NF-кB活性明显升高,NF-кB活性增高可能在GD免疫病理机制中起重要作用。

AIM： To investigate the activity of NF- kB in peripheral blood mononuclear cells （PBMCs） from patients with Graves disease （GD） and the significance in immunopathogenesis of GD. METHODS： Peripheral blood was collected from 22 untreated GD, 20 treated GD with tapazole more than 1 year, and 25 healthy volunteers. PBMCs were isolated from the blood by histopaque - 1077 density - gradient centrifugation. The activity of NF -kB in PBMCs was analyzed using gel electrophoretic mobility shift assay （EMSA）. The contents of IL- 1β, IL-6 and TNF-α were tested by radioimmunoassay. RESULTS ： The activity of NF - kB in PBMCs of untreated GD group was increased remarkably, compared with that in the treated group and control （ P 〈 0. 05 ）. The contents of IL - 1β, IL - 6 and TNF -α in untreated group were significantly higher than those in treated GD and control group （ P 〈 0. 05 ）. A positive correlation between NF - xB activity and IL - 6 level in untreated GD group and treated GD group was observed. CONCLUSION： The activity of NF - kB in PBMCs with GD patients is increased significantly, which might play an important role in the immunopathogenesis of GD.