抗戾饮对呼吸道冠状病毒感染雏鸡血清抗体生成的影响

Effects of Kangliyin on the levels of serum antibody in the chick model of respiratory coronavirus infection

目的:研究抗戾饮对呼吸道冠状病毒——传染性支气管炎病毒(IBV)感染雏鸡模型症状的改善情况,并评价抗戾饮对IBV抗体生成的影响。方法:7d龄雏鸡接种IBV并隔离在适度低温和高湿环境下饲养造模。从攻毒第1d开始,分别灌胃给予模型组、空白对照组无菌生理盐水,治疗组给予抗戾饮水煎剂高中低剂量,阳性对照组给予更昔洛韦。实验时间为5d。观察IBV感染模型临床症状。实验结束后,取肺与气管组织进行病理切片,并采用ELISA方法检测各组雏鸡血清中IBV抗体含量。结果:与模型组相比,抗戾饮组的血清IBV抗体的滴度升高明显(P<0.05),并能有效改善IBV模型的一般症状及肺与支气管组织的病理损伤。结论:抗戾饮可以有效提高模型血清中IBV抗体滴度,改善模型的症状,减轻组织病理损伤,增强IBV感染雏鸡的抗病毒能力。

AIM： To study the effects of Kangliyin compound （KLY, the compound formulation of a Chinese herbal medicine has 15 kinds of ingredients, including artemisia apiacea, baical skullcap root, tangerine peel, pinellia tuber, frutus aurantii immaturus, caulis bambusae in taeniis, tuckahoe, the larva of silkworm with botrytis, turmeric, agastache, rhubarb, cicada ecdysis, and so on. ） treatment on the infectious bronchitis virus （IBV） infection models and to evaluate its effects on the levels of IBV antibody in the serum. METHODS ： Seven - day - old chicks were inoculated with the respiration type of IBV M41 strain. All the chicks were bred in a low temperature and highly moisturized controlled environment. The model group and normal control group were fed with sterile normal saline, while the three treatment groups were administrated with KLY compound. Positive control group was irrigated with ganciclovir. These feeds were lasted for 5 d. The clinical symptoms and the pathological changes of all groups＇ bronchus and lung tissues were examined. The levels of IBV antibody in the serum in each group were also checked by using ELISA method at the end of the study. RESULTS ： Compared with the model group, KLY compound significantly increased the serum titer of IBV antibody in the three treatment groups （ P 〈 0. 05 ） , and improved the general clinical symptoms and respiratory symptoms. The pathological changes of the bronchus and lung tissues in IBV infected model were also improved. CONCLUSION： KLY increases the serum titer of the antibody against IBV and alleviates the pathological damage in the bronchus and lung tissues of IBV model, which would eventually enhances the antiviral capacity of the IBV infected chick.