槲皮素对子宫内膜异位症的抑制作用及机理探讨

Inhibition Effect and Mechanisms of Quercetin on Surgically Induced Endometriosis

目的探讨槲皮素对大鼠子宫内膜异位症模型的异位内膜是否具有抑制作用,以及其抑制作用与热休克蛋白70(HSP70)和血管内皮生长因子(VEGF)表达之间的关系。方法通过手术方法建立大鼠子宫内膜异位症模型,4周后测量异位内膜体积,将造模成功的大鼠分为4组,分别每日灌喂槲皮素、丹那唑、槲皮素+丹那唑以及安慰剂,持续治疗3周后热休克并处死大鼠,测量异位内膜体积并取出异位内膜,检测组织学结构,并采用免疫组化法检测异位内膜组织中HSP70和VEGF的表达。结果与安慰剂相比,槲皮素〔100 mg/(kg.d)〕、丹那唑〔36 mg/(kg.d)〕和槲皮素+丹那唑联合治疗显著减小了异位内膜的体积,但槲皮素、丹那唑及联合治疗后的异位内膜体积差异无统计学意义。与安慰剂和丹那唑相比,槲皮素和联合治疗明显抑制了异位内膜组织中HSP70和VEGF的表达,且在后两个治疗组中HSP70及VEGF的表达差异无统计学意义。结论槲皮素可抑制大鼠子宫内膜异位症模型中异位内膜的生长,并且其抑制效应可能与HSP70和VEGF的表达下降有关,值得进一步研究。

Objective To evaluate the therapeutic effect of quercetin on the rat endometriosis models and the relationship between the inhibition ef/ect and the expression of HSPT0 and VEGF. Methods A surgical model to simulate endometriosis was established and the rats were divided into four groups. After 3 weeks of daily administration of quercetin, dazazol, combined q uercetin＋dazazol, and placebo, the potential rule of quercetin to inhibit endometriosis in rats were evaluated by measuring the implants, examining the histology and detecting the expression of heat shock protein 70 （HSP70） and vascular endothelial growth factor （VEGF） in ectopic endometrium with immunohistochemistry. Results Compared to placebo, quercetin [100 mg/（kg · d）], dazazol [36 mg/（kg · d）], and combined quercetin ＋ dazazol decreased the size of implants significantly respectively, and there was no significant difference among the three groups. HSP70 and VEGF were both significantly reduced by quercetin or combination treatment, but no significant difference was seen between quercetin and combination treatment groups. Conclusion Quercetin inhibits surgically induced endometriosis in rats, and the possible mechanism is to inhibit the expression of HSP70 and VEGF.