日本血吸虫童虫cDNA文库免疫学筛选及阳性克隆的初步鉴定

Immunoscreening and Identification of Schistosoma japonicum Juvenile cDNA Library

用日本血吸虫感染14d的小鼠血清免疫筛选日本血吸虫童虫cDNA文库,将获得的7个阳性克隆进行核苷酸序列同源性分析,结果显示其中一个克隆所测序列与日本血吸虫HSP70有很高的同源性(分值score=650),另有2个克隆所测序列分别与已报道的日本血吸虫FABP(score=229)和含锌指结构的CDGSH型蛋白样蛋白(score=246)明显同源,其余4个未找到已知的同源序列,为新基因。4个新基因序列已被GenBank接受(登录号为EU121231、202646、202647和202648)。

The cDNA library of Schistosoma japonicum （Sj） juveniles was immunoscreened with the anti-serum from day 14 post-infection mice. The inserts of the seven positive clones were sequenced and analyzed for their homology in GenBank database. Results showed that one was highly homologous to the SjHSP70 （score=650）, two were significantly homologous to the SjFABP（score=229） and Sj CDGSH-type Zn finger-containing protein-like protein（score= 246）, and the other four were not homologous to genes in GenBank and thus identified as Sj novel genes. The sequences of the novel genes were submitted to GenBank and the accession numbers were obtained （EU121231, 202646, 202647 and 202648）.