模板纯化对HBV拉米呋啶耐药突变检测的检出率影响

Effect of purification methods of serum HBV DNA on sensitivity of detection of HBV lamivuding resistance

目的:研究模板纯化与否对HBV拉米呋啶耐药突变检测的检出率影响。方法:采用离心柱纯化法及直接煮沸裂解法分别处理HBV DNA定量小于104IU/ml的60例患者血清标本,用同一HBV拉米呋啶耐药突变检测荧光PCR试剂盒检测,比较其检出率。结果:HBV DNA定量小于104IU/ml的60例患者血清标本,离心柱纯化组和煮沸裂解组的检出率分别为68.3%和38.3%。结论:低病毒载量的HBV血清样品经离心柱纯化模板后可以明显提高HBV拉米呋啶耐药突变检测的检出率。

Objective：To investigate the effect of purification methods of plasma HBV DNA on sensitivity of detection of HBV lamivuding resistance. Methods ：Serum HBV DNA template was prepared by both spin column purification and directly boiling method and HBV Serum samples with 60 clinical samples of 10^4 IU/ml. The effect of purification methods on the assay sensitivity was evaluated by the domestic commercial YMDD mutation kit. Results： The sensitivity of spin column method and boiling method are 68.3% and 38.3% , respectively. Conclusion：The purification of HBV DNA in serum sample by spin column method can remarkably improve the assay sensitivity of HBV lamivuding resistance.