HIV抗体初筛阳性标本检测结果分析

Analysis of confirmatory test results of anti-HIV screening positive samples

目的：探讨HIV抗体初筛实验结果与确认实验结果之间的关系,为艾滋病检测提供科学依据。方法：2003—2007年所有标本采用ELISA及快速检测试剂进行初筛，初筛阳性标本送省疾控中心用蛋白印迹（WB）法确认。结果：340份HIV抗体初筛阳性标本，经蛋白印迹（WB）法确认阳性79份，其中初筛实验两阳或两阳以上标本确认阳性率为86．8％；一阳一阴标本确认率为0％。结论：利用二种不同原理的初筛试剂检测HIV抗体与WB确认试验的检测结果具有很好的相关关系，在对个体进行诊断时，为慎重起见，必须经WB法确认。

Objective：To study the relationship between the resuhs of the primary screening test and the confirmatory test and provide a scientific evidence for the detection of HIV infection. Methods：The ELISA kit and rapid detection reagents were used for screening blood samples from 2003 to 2007 with HIV antibody and all the positive results must be confirmed by western blot assay. Results：Among 340 HIV antibody-positive samples from primary screening test ,79 samples were confirmed to have been infected with HIV by western blot assay. The confirmed positive rate of samples whose primary screening test results were all positive by using at least two kinds of screening reagents for anti - HIV detection was 86. 8%, while that of samples whose primary screening test results were inconsistent by using two kinds of screening reagents with different principles was 0%. Conclusion ： The results between the primary screening test for anti - HIV detection by using two kinds of screening reagents with different principles and the western blot assay are correlative. Confirmation of HIV infection relies on WB.