大酱引发的食物中毒中检出A型肉毒毒素的报告

Soybean caused by food poisoning in report of detection of type A botulinum toxin

目的:探讨1例由食入大酱而引发的食物中毒报告中检出A型肉毒毒素。方法:采用A型肉毒毒素快速检测试剂(免疫胶体金法)进行初筛,再按照GB/T4789.12-2003国标方法,采用小白鼠腹腔注射方法进行验证试验。结果:经过初筛及验证试验,在大酱中检出了A型肉毒毒素。结论:通过免疫胶体金法及国标方法证实了大酱引发的食物中毒中A型肉毒毒素的存在,表明了由于食入被肉毒梭菌污染的不洁食品等仍是引发食物中毒的主要原因。

Objective：To discuss 1 example food poisoning caused by the ingestion of soybean paste and detect type A botulinum toxin. Methods：Used botulinum toxin type A rapid detection kit （immunogold method） for screening, and then confirmd by GB/T4789. 12-2003 in accordance with national standard methods, using mice injected test methods. Results： After screening and confirmatory tests, detectd type A botulinum toxin in soybean paste. Conclusion：Immunogold method and national standard method confirmed the existence of botulinum toxin type A and indicated that ingestion of contaminated by clostridium botulinum and other unclean food is still the main cause of food poisoning reasons.