冬虫夏草菌丝体的安全性毒理学评价

Toxicological assessment of coudyceps sinensis berkely sacc

目的：为冬虫夏草菌丝体安全性评价提供毒理学依据。方法：按卫生部GB．15193～94《食品安全性毒理学评价程序和方法》进行急性毒性、小鼠骨髓细胞微核试验、小鼠精子畸形试验、Ames试验和大鼠30d喂养试验。结果：冬虫夏草菌丝体对雌、雄小鼠和雌、雄大鼠经口LD50均大于20．0g／kg体重，属无毒级；3种致突变试验均未见致突变作用；对大鼠30d喂养试验结果，3个剂量组大鼠各项指标与对照组比较均无显著性差异。结论：冬虫夏草菌丝体属无毒级；未见潜在致突变作用；对大鼠30d喂养试验，未见明显毒性反应。在本实验条件下，得出冬虫夏草菌丝体对大鼠的未观察到有害作用最大剂量为5．00g／kg体重。

Objective：To provide basis for the toxicological, assessment of coudyceps sinensis berkely sacc（CSBS）. Methods： Acute toxicity test, bone marrow cell micronucleus test, mice sperm abnormality test, Ames test and thirty days feeding study were conducted according to GB - 15193 - 94. Results： CSBS on the per - oral LDS0 in mice and in rats were all 〉 20. 0 g/kg body weight （BW）. It belonged to non -toxic. CSBS on the bone marrow cell mieronueleus test, mice sperm abnormality test and Ames test were all not mutation effect. On the thirty days feeding study in rats ： All observed - index of the rats fed CSBS were all not significant with the control＇s. Conclusion： CSBS is belong to non -toxic. It has not mutation effect. No -observed -adverse - effect - level （NOAEL） of CSBS on rats is 5.0 g/kg · BW.