运动预处理对力竭运动诱导的大鼠海马细胞凋亡的影响

Effect of Exercise Preconditioning on Exhaustive Exercise Induced the Apoptosis of Neurons in Rats'Hippocampus

目的:建立SD大鼠运动预处理模型和一次性力竭运动模型,探讨运动预处理对一次性力竭运动诱导的海马细胞凋亡的影响及其可能机制;方法:将24只雄性SD大鼠随机分为对照组(C组),力竭运动组(EE组),运动预处理组(EP组),EP组进行持续4周的中等负荷游泳训练后,EE组和EP组负自身体重的3%进行一次性力竭游泳,用免疫组织化学法检测Bcl-2和Bax基因的蛋白表达;结果:EP组大鼠海马的Bcl-2阳性表达强度显著升高,EE组大鼠海马的Bax阳性表达强度显著升高;结论:1)一次性力竭运动可引起大鼠海马神经元凋亡;2)运动预处理能减少力竭运动诱导的大鼠海马细胞凋亡;3)凋亡调控基因Bcl-2、Bax对运动引起的细胞凋亡有明显的调控作用。

Purpose：The experiment upbuilds rats ’ exercise preconditioning models and exhaustive exercise models, to discuss the influence of exercise preconditioning on exhaustive exerciseinduced the apoptosis of neurons in rats ＇ hippocampus and the mechanism of the effects. Meth od： 24 SD rats were divided into three groups at random control group （group C）, exhaustive exercise group （group EE） and exercise preconditioning group （group EP）. First, group EP carries out moderate exercise preconditioning for 4 weeks, then group EE and EP carry out an exhaustive swimming. Bcl-2 and Bax albumen were examined with the immunohistochemical method. Result： The intolerant expression intensity of Bcl-2 of the hippoeampal of rats of group EP was increased. The intolerant expression intensities of Bax of the hippocampal of rats of Group EE were also obviously increased. Conclusion： 1. Exhaustive exercise increases the apoptosis of the hippocampal neuron cells. 2. It can reduce exhaustive exercise induced the apop tosis of the hippocampal neuron cells by moderate exercise preconditioning. 3. Protein Bcl-2 and Bax have great adjusting and controlling functions related to the apoptosis of cells which was induced by exercise.